Novel role of cPLA2α in membrane and actin dynamics
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- Moes, M., Boonstra, J. & Regan-Klapisz, E. Cell. Mol. Life Sci. (2010) 67: 1547. doi:10.1007/s00018-010-0267-0
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Actin-directed processes such as membrane ruffling and cell migration are regulated by specific signal transduction pathways that become activated by growth factor receptors. The same signaling pathways that lead to modifications in actin dynamics also activate cPLA2α. Moreover, arachidonic acid, the product of cPLA2α activity, is involved in regulation of actin dynamics. Therefore, it was investigated whether cPLA2α plays a role in actin dynamics, more specifically during growth factor-induced membrane ruffling and cell migration. Upon stimulation of ruffling and cell migration by growth factors, endogenous cPLA2α and its active phosphorylated form were shown to relocate at protrusions of the cell membrane involved in actin and membrane dynamics. Inhibition of cPLA2α activity with specific inhibitors blocked growth factor-induced membrane and actin dynamics, suggesting an important role for cPLA2α in these processes.
KeywordscPLA2α Actin Arachidonic acid Membrane dynamics Ruffles Lamellae Leading edge Migration
Cytosolic PLA2 alpha
Human umbilical vein endothelial cells
Actin plays an essential role in a wide variety of dynamic events in mammalian cells, amongst them cell motility, cytokinesis, and cell–cell and cell–matrix interactions [1, 2, 3, 4]. Actin dynamics are determined by the activities of a large number of actin-binding proteins. Extracellular stimuli, like growth factors and extracellular matrix components, stimulate intracellular signal transduction cascades that activate actin-binding proteins resulting in changes in local actin dynamics. These changes result in morphological changes like the formation of membrane ruffles and leading edges. In addition to changed actin dynamics upon growth factor addition or cell spreading, many signal transduction components are bound to actin, indicating the mutual relationship [5, 6, 7].
An important molecule involved in the regulation of cell metabolism is the unsaturated fatty acid arachidonic acid, which serves as a precursor for leukotriene and prostaglandin synthesis. However, arachidonic acid release has also been related to the regulation of actin dynamics . In HeLa cells, an increase in arachidonic acid production was shown after cell attachment. This results in both actin polymerization and actin bundling via two distinct pathways involving lipoxygenase and cyclooxygenase [9, 10]. These enzymes metabolize arachidonic acid, resulting in the release of lipid second messengers that activate actin-binding proteins and consequently cause a change in actin dynamics. Additional to its role in cell spreading and immobilization, arachidonic acid was also shown to stimulate cell motility via the production of prostaglandins by cyclooxygenase. Prostaglandins play a role in actin cytoskeletal regulation and migration in a variety of ways. For example, stimulation of cells by chemokines resulted in an increase of prostaglandins and consequently in an increase of actin polymerization . Moreover, several forms of stimulated migration require arachidonic acid metabolites [12, 13]. In addition, dissolution of adhesion structures like podosomes is dependent on the production of prostaglandins . The role of arachidonic acid-mediated signaling in the regulation of small GTPases that modulate actin remodeling has been illustrated in several studies. In Swiss 3T3 fibroblasts, various leukotrienes induce stress fiber formation via a Rho-dependent process . In Rat2 fibroblasts, arachidonic acid induces membrane ruffling and this is prevented when a dominant-negative mutant of Rac1 (Rac1N17) is expressed, indicating that Rac1 is activated downstream of arachidonic acid-mediated signaling . Since Rac was also shown to act upstream of arachidonic acid release , it is possible that arachidonic acid and Rac1 act in a positive feedback loop.
The release of arachidonic acid in cells results from cleavage of membrane phospholipids at the sn-2 position by phospholipases A2 (PLA2). The large family of PLA2 consists of several sub-families like the secretory PLA2s (sPLA2), the intracellular calcium independent PLA2s (iPLA2), and the group of cytosolic PLA2s (cPLA2) including cPLA2α . Interestingly, cPLA2α is highly specific for phospholipids carrying arachidonic acid at the sn-2 position and is therefore considered as the rate-limiting enzyme in receptor-mediated arachidonic acid release. cPLA2α requires Ca2+ to translocate from the cytosol to the membrane through its CaLB (calcium-lipid binding) domain, although Ca2+ is not directly involved in catalysis . The activation of cPLA2α results from a phosphorylation by MAP kinase (for review, see ). This MAP kinase on its turn is activated by the same signal transduction cascades (growth factors and extracellular matrix components) that lead to modifications in actin dynamics. Therefore, it is tempting to suggest that cPLA2α is one of the key enzymes involved in local actin dynamics that are involved in the formation of membrane ruffles, and in cell motility.
Therefore, we have studied the localization of cPLA2α in fibroblasts during stimulation with platelet-derived growth factor (PDGF). Stimulation with PDGF introduces circular dorsal ruffles and lamellipodia. Here, we show that endogenous cPLA2α and its active, phosphorylated form are localized at the cell membrane in response to PDGF stimulation. They localize specifically to areas at the cell membrane that are reshaped by actin during PDGF stimulation. Furthermore, endogenous cPLA2α and its active phosphorylated form are shown to localize at leading edges in primary human umbilical vein endothelial cells (HUVECs) and migrating mouse fibroblasts. Interestingly, inhibition of cPLA2α activity with specific inhibitors blocked PDGF-induced membrane and actin dynamics, suggesting a role for cPLA2α in these processes.
Materials and methods
Human umbilical vein endothelial cells were isolated according to Jaffe  and used from passage 1 to 4. HUVECs were cultured onto fibronectin-coated surfaces and grown in endothelial basal medium (EBM-2) supplemented with 2% fetal bovine serum (FBS) (Gibco, Paisley, UK), endothelial cell growth supplements (EGM-2) (Cambrex, NJ, USA), and 5 mM l-glutamine (Invitrogen). C3H10T1/2 mouse fibroblasts were cultured in HEPES (25 mM) buffered Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 7.5% FBS and 5 mM l-glutamine. Cells were grown at 37°C (humidified atmosphere, 5% CO2).
HUVECs were seeded on glass coverslips at 10,000 cells/cm2, coated with fibronectin and allowed to grow for 2 or more days to obtain sub-confluent monolayers for localization studies and confluent monolayers for the scratch assays.
Fibroblasts were plated on glass coverslips at 12,000 cells/cm2 and allowed to grow for 24 h. For PDGF stimulation experiments, cells were subsequently growth factor-starved for 24 h at 37°C and stimulated with 20 ng/ml of PDGF-BB (Upstate, Hampshire, UK). Treatment with cPLA2α inhibitors Pyrrolidine-2 (2 μM) and Wyeth-1 (5 μM) was performed in serum-free medium. Both inhibitors were kind gifts from Prof. Dr. M. Gelb (University of Washington, Seattle, USA).
Cells were fixed in 3.2% formaldehyde for 15 min, washed twice with PBS, permeabilized for 5 min in PBS containing 0.2% Triton X-100, followed by two washes in PBS, and incubated for 10 min with 50 mM glycine in PBS. After washing twice with PBS containing 0.2% gelatin, cells were incubated overnight with goat anti-cPLA2 (Santa Cruz; sc-1724) or for 1 h with rabbit anti-phospho-cPLA2 (ser505) (Santa Cruz; sc-34391). Then, the cells were washed six times with PBS containing 0.2% gelatin and incubated for 1 h with tetramethylrhodamine-5-(and-6)-isothiocyanate (TRITC)-conjugated phalloidin (Sigma) combined with goat-anti-rabbit-Alexa488 or donkey-anti-goat-Alexa488 (Molecular Probes) and washed six times PBS containing 0.2% gelatin. In some samples, this was followed by a DAPI staining for 10 min. Finally, cells were mounted in Mowiol-DABCO.
Transfections with GFP-cPLA2α were performed using Lipofectin (Invitrogen) according to the manufacturer’s instructions. This construct, kindly provided by Dr. C. Lenoir (Inserm Unit 538, Paris, France), was checked by sequencing.
HUVECs were seeded on fibronectin-coated glass coverslips at 10,000 cells/cm2 (~10–20% confluency) and allowed to grow for 7 days with regular change of medium. Cells were gently scratched from the substrate using a pipette tip. The remaining cells were washed with culture medium and allowed to reoccupy the wounded area for various time intervals. Mouse C3H10T1/2 fibroblasts were seeded at 20,000 cells/cm2 and allowed to grow to confluency before performing the scratch assay as described above.
Acquisition of images
Samples were studied with a Leitz microscope (Orthoplan Flu 043944) equipped with Leitz oil objectives ×40 NA 1.3 and ×63 NA 1.4. Images were acquired using a Leica CCD camera (model DC350F; Leica Microsystems) using Leica Image Manager 50 software. Confocal pictures were obtained using a Zeiss CLSM (Pascal 510) equipped with Zeiss water objectives (×40 NA 1.3 and ×63 NA 1.4). Images were processed with Adobe Photoshop 8.0.
cPLA2α is recruited to PDGF-induced dorsal membrane ruffles and lamellae in C3H10T1/2 fibroblasts
In addition to the formation of membrane ruffles, C3H10T1/2 cells also form lateral lamellae upon addition of PDGF (Fig. 1n). Whether the cells form lamellae or circular ruffles is determined by the PDGF concentration used. At concentrations >20 ng/ml, abundant circular ruffling takes place, whereas at concentrations in the range of 1–5 ng/ml, less circular dorsal ruffles occur and more lamellae are formed. The formation of lamellae is related to cell spreading and/or cell motility. As clearly shown in Fig. 1n, treatment of cells with 20 ng/ml PDGF for 10 min at 37°C results in the formation of lamellae characterized by a lateral band of newly formed F-actin (Fig. 1n) in which cPLA2α accumulates (Fig. 1m), while the perinuclear localization of cPLA2α, as observed in untreated cells (Fig. 1j), disappeared. Together these data clearly demonstrate that PDGF-induced actin remodeling is accompanied by a recruitment of cPLA2α to sites of new actin polymerization and membrane dynamics.
cPLA2α is localized at leading edges of migrating mouse fibroblasts
cPLA2α is localized at leading edges of migrating primary human endothelial cells
To determine whether the recruitment of cPLA2α at sites of actin polymerization as observed in fibroblasts is a conserved phenomenon, the relationship between cPLA2α and F-actin was studied in primary endothelial cells of human origin, i.e. HUVECs. It has been demonstrated that cPLA2α translocates to the Golgi complex in response to confluency in HUVECs [25, 26]. A scratch assay was performed on a confluent monolayer of HUVECs and the localization of cPLA2α and F-actin was examined. Immediately after scratch, cPLA2α was localized at the Golgi apparatus in all cells (lining the wound or not) (Fig. 2h). Four hours after the scratch, cells located at the border of the wound displayed protrusions of the plasma membrane in which cPLA2α was detected and where cPLA2α locally co-localized with F-actin (data not shown). Subsequently migrating cells reoccupy the wounded area. Twenty-two hours after scratch, migrating cells filled up the wounded area (Fig. 2j, k, l) and displayed cPLA2α staining at the cell membrane (Fig. 2k) and leading edge (Fig. 2k insert), where it locally colocalizes with F-actin. In contrast to confluent cells (Fig. 2h), in the wounded area, cPLA2α is localized primarily in the cytoplasm and also at the cell periphery (Fig. 2k). This latter localization may both be related to cell spreading and cell migration.
Phosphorylated cPLA2α is present in circular dorsal ruffles and leading edges
Inhibition of cPLA2α enzymatic activity prevents the formation of PDGF-induced circular ruffles and lamellae
Arachidonic acid release has been demonstrated to play a role in actin polymerization. Since activation of cPLA2α results in the preferential release of arachidonic acid, it is tempting to suggest that cPLA2α is a key enzyme involved in the regulation of local actin dynamics. Therefore, we have studied the localization of cPLA2α in fibroblasts and HUVECs in relation to induced actin dynamics. Endogenous cPLA2α was shown to localize at the plasma membrane in both migrating mouse fibroblasts and migrating HUVEC. Moreover, the phosphorylated form of cPLA2α (on Ser505) was found at the plasma membrane in both cell types. Both cPLA2α and phospho-S505-cPLA2α localize at protrusions of the cell membrane that show active actin and membrane remodeling such as circular dorsal ruffles, lamellipodia, and leading edges. Interfering with the activity of cPLA2α in mouse fibroblasts resulted in the blocking of PDGF-induced actin and membrane dynamics.
cPLA2α is present at the cell membrane in a spatio-temporal manner. Upon PDGF stimulation, cPLA2α docks at specific sites at the cell membrane, namely at lamellipodia and ruffles. Upon stimulation of migration, cPLA2α docks at the leading edge of motile cells. Also here, the localization of cPLA2α at the cell membrane is local and temporal. This indicates that its docking at the cell membrane is tightly regulated. It was suggested that cPLA2α interacts with membranes via PtdIns(4,5)P2. It was shown that PtdIns(4,5)P2 stimulates cPLA2α activity independent of calcium suggesting an interaction between PtdIns(4,5)P2 and cPLA2α allowing cPLA2α to reach its substrate at membranes . PtdIns(4,5)P2 is primarily located in the cytoplasmic leaflet of the plasma membrane. Interestingly, it has been demonstrated that a variety of signal transduction proteins, including growth factor receptors, reside in areas of high actin polymerization, such as membrane ruffles . Receptor activation can increase or decrease local PtdIns(4,5)P2 concentrations via the local activation of kinases and phosphatases like PI5K, phospholipase C, phospholipase D, and PI3K . By doing so, receptor activation might also regulate the docking of cPLA2α at the cell membrane upon growth factor stimulation. Interestingly PtdIns(4,5)P2 was shown to be enriched locally at the leading edge  and phosphatidylinositide metabolism was shown to be involved in ruffle formation [34, 35, 36].
The local presence of cPLA2α and its phosphorylated active form at sites of actin and membrane remodeling indicates a role for cPLA2α in regulating membrane dynamics. This is also suggested by blocking of PDGF-induced actin and membrane dynamics after exposure to the cPLA2α inhibitors Pyrrolidine-2 and Wyeth-1. Of note, these inhibitors also block the enzymatic activity of another cPLA2 from group IV, namely cPLA2ζ . This enzyme can release arachidonic acid from membrane phospholipids but, contrary to cPLA2α, it does not have specificity for arachidonic acid versus other fatty acids . However, in C3H10T1/2, cPLA2ζ is not expressed, as confirmed by RT-PCR (our unpublished data). This observation strengthens our inhibitors-based results, reinforcing the idea that cPLA2α plays a major role in regulating actin dynamics.
The local presence of the active form of cPLA2α might indicate a local production of arachidonic acid by cPLA2α. Subsequently, arachidonic acid might affect locally the behavior of actin via arachidonic metabolites. Arachidonic acid release has been previously related to the behavior of actin. During cell spreading, actin is changing the cytoskeleton and thereby changing the cell morphology. In HeLa cells, an increase in arachidonic acid production was shown after cell attachment. This results in both actin polymerization and actin bundling via two distinct pathways involving lipoxygenase and cyclooxygenase [9, 10]. Lipoxygenase metabolizes arachidonic acid and via a cascade of lipid second messengers protein kinase C epsilon (PKCε) is subsequently activated which triggers actin polymerization leading to cell spreading. Cyclooxygenase generates prostaglandins resulting in the activation of cyclic AMP-dependent protein kinase A (PKA) that induces actin bundling. Moreover, PKA was shown to play a role in the actin cytoskeleton regulation in a variety of ways (for review, see ). So, an increase of arachidonic acid causes an increase in actin polymerization and bundling during cell spreading by activating actin-binding proteins like PKA and PKCε. Furthermore, arachidonic acid and some of its metabolites can activate signaling pathways leading to the activation of small GTPases such as Rac1 and Rho [15, 16], ultimately resulting in actin remodeling. It has long been known that arachidonic acid and some of its metabolites can bind directly to and regulate the activity of specific GTPase-activating proteins (GAPs), which are regulators of small GTPases [38, 39]. Altogether, it is tempting to suggest that the local production of arachidonic acid and of its metabolites could trigger signaling pathways resulting in the local remodeling of actin.
In addition, cPLA2α is able to change the local characteristics of the plasma membrane by interacting with membrane phospholipids. cPLA2α-mediated hydrolysis of cellular membranes results in the production of lysophospholipids within the lipid bilayer and the lysophospholipids thus produced change the plasma membrane microviscosity. A change in the plasma membrane microviscosity by lysophospholipids was shown to affect cell migration . Plasma membrane microviscosity has an important role on actin dynamics and thereby regulates cell motility [40, 41]. It was suggested that an increase in the plasma membrane microviscosity in lamellipodia might result in a local increase of actin-directed movement . Our results show active cPLA2α at sites of active actin remodeling, and cPLA2α might here locally direct the behavior of actin by changing the plasma membrane microviscosity.
Altogether, our data show that cPLA2α is specifically recruited at sites of actin and membrane remodeling in different cellular processes. Since this phenomenon occurs in mouse fibroblasts and in primary human endothelial cells, it is likely to represent a general property of cPLA2α. However, further research is required to fully elucidate the role of cPLA2α in the above-mentioned processes.
We like to thank Prof. M. Gelb for supplying the cPLA2α inhibitors. We thank C. Lenoir for supplying the GFP-cPLA2α constructs. We thank Bart de Haan and Miriam Langelaar-Makkinje for isolating HUVECs. We thank José Bijvelt and Mohammed El Khattabi for their help with the RT–PCR of cPLA2α and cPLA2ζ. This work is subsidized by the Dutch Space Organization (SRON, grant MG-059).
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