Cellular and Molecular Life Sciences

, Volume 66, Issue 6, pp 1039–1056

DNA Repair in Mammalian Cells

DNA double-strand break repair: how to fix a broken relationship
Multi-author Review

DOI: 10.1007/s00018-009-8740-3

Cite this article as:
Pardo, B., Gómez-González, B. & Aguilera, A. Cell. Mol. Life Sci. (2009) 66: 1039. doi:10.1007/s00018-009-8740-3


DNA double-strand breaks (DSBs) arise in cells from endogenous and exogenous attacks on the DNA backbone, but also as a direct consequence of replication failures. Proper repair of all these DSBs is essential for genome stability. Repair of broken chromosomes is a challenge for dividing cells that need to distribute equal genetic information to daughter cells. Consequently, eukaryotic organisms have evolved multi-potent and efficient mechanisms to repair DSBs that are primarily divided into two types of pathways: nonhomologous end joining (NHEJ) and homologous recombination (HR). Here we briefly describe how eukaryotic cells sense DSBs and trigger cell cycle arrest to allow repair, and we review the mechanisms of both NHEJ and HR pathways and the choice between them. (Part of a Multi-author Review)


DNA repairgenome stabilitycheckpointnonhomologous end joininghomologous recombinationrepair pathway choice

Copyright information

© Birkhäuser Verlag, Basel 2009

Authors and Affiliations

  1. 1.Centro Andaluz de Biología Molecular y Medicina Regenerativa CABIMERUniversidad de Sevilla-CSICSevillaSpain