Cellular and Molecular Life Sciences

, Volume 66, Issue 5, pp 933–947

Different carbon sources affect lifespan and protein redox state during Saccharomyces cerevisiae chronological ageing

Authors

    • Dipartimento di Scienze Biochimiche Università degli Studi di Firenze
  • A. Carpentieri
    • Dipartimento di Chimica Organica e BiochimicaUniversità di Napoli Federico II
  • A. Amoresano
    • Dipartimento di Chimica Organica e BiochimicaUniversità di Napoli Federico II
  • T. Gamberi
    • Dipartimento di Scienze Biochimiche Università degli Studi di Firenze
  • C. De Filippo
    • Dipartimento di Farmacologia Preclinica e ClinicaUniversità degli studi di Firenze
  • L. Rizzetto
    • Dipartimento di Farmacologia Preclinica e ClinicaUniversità degli studi di Firenze
    • Dipartimento di Genetica, Antropologia, ed EvoluzioneUniversitá di Parma
  • M. Biagini
    • Dipartimento di Scienze Biochimiche Università degli Studi di Firenze
  • P. Pucci
    • Dipartimento di Chimica Organica e BiochimicaUniversità di Napoli Federico II
  • A. Modesti
    • Dipartimento di Scienze Biochimiche Università degli Studi di Firenze
Research Article

DOI: 10.1007/s00018-009-8574-z

Cite this article as:
Magherini, F., Carpentieri, A., Amoresano, A. et al. Cell. Mol. Life Sci. (2009) 66: 933. doi:10.1007/s00018-009-8574-z

Abstract.

In this study, a proteomic approach that combines selective labelling of proteins containing reduced cysteine residues with two-dimensional electrophoresis/mass spectrometry was used to evaluate the redox state of protein cysteines during chronological ageing in Saccharomyces cerevisiae. The procedure was developed on the grounds that biotinconjugated iodoacetamide (BIAM) specifically reacts with reduced cysteine residues. BIAM-labelled proteins can then be selectively isolated by streptavidin affinity capture. We compared cells grown on 2% glucose in the exponential phase and during chronological ageing and we found that many proteins undergo cysteine oxidation. The target proteins include enzymes involved in glucose metabolism. Both caloric restriction and growth on glycerol resulted in a decrease in the oxidative modification. Furthermore, in these conditions a reduced production of ROS and a more negative glutathione half cell redox potential were observed.

Keywords.

Yeasttwo-dimensional electrophoresischronological ageingcaloric restrictionproteins oxidation

Supplementary material

18_2009_8574_MOESM1_ESM.pdf (60 kb)
(PDF 56 kb)

Copyright information

© Birkhäuser Verlag, Basel 2009