Cellular and Molecular Life Sciences

, Volume 67, Issue 4, pp 655–669

Intra-brain microinjection of human mesenchymal stem cells decreases allodynia in neuropathic mice

  • Dario Siniscalco
  • Catia Giordano
  • Umberto Galderisi
  • Livio Luongo
  • Nicola Alessio
  • Giovanni Di Bernardo
  • Vito de Novellis
  • Francesco Rossi
  • Sabatino Maione
Research Article

DOI: 10.1007/s00018-009-0202-4

Cite this article as:
Siniscalco, D., Giordano, C., Galderisi, U. et al. Cell. Mol. Life Sci. (2010) 67: 655. doi:10.1007/s00018-009-0202-4

Abstract

Neuropathic pain is a very complex disease, involving several molecular pathways. Current available drugs are usually not acting on the several mechanisms underlying the generation and propagation of pain. We used spared nerve injury model of neuropathic pain to assess the possible use of human mesenchymal stem cells (hMSCs) as anti-neuropathic tool. Human MSCs were transplanted in the mouse lateral cerebral ventricle. Stem cells injection was performed 4 days after sciatic nerve surgery. Neuropathic mice were monitored 7, 10, 14, 17, and 21 days after surgery. hMSCs were able to reduce pain-like behaviors, such as mechanical allodynia and thermal hyperalgesia, once transplanted in cerebral ventricle. Anti-nociceptive effect was detectable from day 10 after surgery (6 days post cell injection). Human MSCs reduced the mRNA levels of the pro-inflammatory interleukin IL-1β mouse gene, as well as the neural β-galactosidase over-activation in prefrontal cortex of SNI mice. Transplanted hMSCs were able to reduce astrocytic and microglial cell activation.

Keywords

Neuropathic painHuman mesenchymal stem cellsStem cell transplantationRegenerative medicine

Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2009

Authors and Affiliations

  • Dario Siniscalco
    • 1
  • Catia Giordano
    • 1
  • Umberto Galderisi
    • 2
  • Livio Luongo
    • 1
  • Nicola Alessio
    • 2
    • 3
  • Giovanni Di Bernardo
    • 2
  • Vito de Novellis
    • 1
  • Francesco Rossi
    • 1
  • Sabatino Maione
    • 1
  1. 1.Division of Pharmacology “L. Donatelli”, Department of Experimental MedicineSecond University of NaplesNaplesItaly
  2. 2.Division of Biotechnology and Molecular Biology “A. Cascino”, Department of Experimental MedicineSecond University of NaplesNaplesItaly
  3. 3.University of SassariSassariItaly