Research Article

Cellular and Molecular Life Sciences

, Volume 67, Issue 4, pp 611-628

First online:

The metazoan ATAC and SAGA coactivator HAT complexes regulate different sets of inducible target genes

  • Zita NagyAffiliated withDepartment of Functional Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104, INSERM U 964, Université de Strasbourg
  • , Anne RissAffiliated withDepartment of Functional Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104, INSERM U 964, Université de Strasbourg
  • , Sally FujiyamaAffiliated withInstitute of Molecular and Cellular Biosciences, The University of Tokyo
  • , Arnaud KrebsAffiliated withDepartment of Functional Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104, INSERM U 964, Université de Strasbourg
  • , Meritxell OrpinellAffiliated withDepartment of Functional Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104, INSERM U 964, Université de Strasbourg
  • , Pascal JansenAffiliated withDepartment of Molecular Biology, NCMLS 274, Radboud University Nijmegen
  • , Adrian CohenAffiliated withDepartment of Molecular Biology, NCMLS 274, Radboud University Nijmegen
  • , Henk G. StunnenbergAffiliated withDepartment of Molecular Biology, NCMLS 274, Radboud University Nijmegen
  • , Shigeaki KatoAffiliated withInstitute of Molecular and Cellular Biosciences, The University of Tokyo
    • , Làszlò ToraAffiliated withDepartment of Functional Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104, INSERM U 964, Université de Strasbourg Email author 

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Abstract

Histone acetyl transferases (HATs) play a crucial role in eukaryotes by regulating chromatin architecture and locus-specific transcription. The GCN5 HAT was identified as a subunit of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) multiprotein complex. Vertebrate cells express a second HAT, PCAF, that is 73% identical to GCN5. Here, we report the characterization of the mammalian ATAC (Ada-Two-A-Containing) complexes containing either GCN5 or PCAF in a mutually exclusive manner. In vitro ATAC complexes acetylate lysine 14 of histone H3. Moreover, ATAC- or SAGA-specific knock-down experiments suggest that both ATAC and SAGA are involved in the acetylation of histone H3K9 and K14 residues. Despite their catalytic similarities, SAGA and ATAC execute their coactivator functions on distinct sets of inducible target genes. Interestingly, ATAC strongly influences the global phosphorylation level of histone H3S10, suggesting that in mammalian cells a cross-talk exists linking ATAC function to H3S10 phosphorylation.

Keywords

ADA2a GCN5 ATAC2 Histone acetyltransferase Immediate early gene PCAF H3S10P