Cellular and Molecular Life Sciences

, Volume 65, Issue 3, pp 414–444

Histone chaperones: 30 years from isolation to elucidation of the mechanisms of nucleosome assembly and disassembly

Review

DOI: 10.1007/s00018-007-7305-6

Cite this article as:
Eitoku, M., Sato, L., Senda, T. et al. Cell. Mol. Life Sci. (2008) 65: 414. doi:10.1007/s00018-007-7305-6

Abstract.

Some three decades have passed since the discovery of nucleosomes in 1974 and the first isolation of a histone chaperone in 1978. While various types of histone chaperones have been isolated and functionally analyzed, the elementary processes of nucleosome assembly and disassembly have been less well characterized. Recently, the tertiary structure of a hetero-trimeric complex composed of the histone chaperone CIA/ASF1 and the histone H3-H4 dimer was determined, and this complex was proposed to be an intermediate in nucleosome assembly and disassembly reactions. In addition, CIA alone was biochemically shown to dissociate the histone (H3-H4)2 tetramer into two histone H3-H4 dimers. This activity suggested that CIA regulates the semi-conservative replication of nucleosomes. Here, we provide an overview of prominent histone chaperones with the goal of elucidating the mechanisms that preserve and modify epigenetic information. We also discuss the reactions involved in nucleosome assembly and disassembly.

Keywords.

Histones chaperones nucleosome assembly nucleosome disassembly mechanisms overview 

Copyright information

© Birkhaueser 2007

Authors and Affiliations

  1. 1.Laboratory of Developmental Biology, Institute of Molecular and Cellular BiosciencesUniversity of TokyoTokyoJapan
  2. 2.Biological Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST)TokyoJapan

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