Review

Cellular and Molecular Life Sciences

, 64:892

First online:

Structure, mechanism and catalytic duality of thiamine-dependent enzymes

  • R. A. W. FrankAffiliated withDepartment of Biochemistry, University of CambridgeWellcome Trust Sanger Institute
  • , F. J. LeeperAffiliated withDepartment of Chemistry, University of Cambridge
  • , B. F. LuisiAffiliated withDepartment of Biochemistry, University of Cambridge Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract.

Thiamine is an essential cofactor that is required for processes of general metabolism amongst all organisms, and it is likely to have played a role in the earliest stages of the evolution of life. Here, we review from a structural perspective the enzymatic mechanisms that involve this cofactor. We explore asymmetry within homodimeric thiamine diphosphate (ThDP)-dependent enzyme structures and discuss how this may be correlated with the kinetic properties of half-of-the-sites reactivity, and negative cooperativity. It is likely these structural and kinetic hallmarks may arise through reciprocal coupling of active sites. This mode of communication between distant active sites is not unique to ThDP-dependent enzymes, but is widespread in other classes of oligomeric enzyme. Thus, it appears likely to be a general phenomenon reflecting a powerful mechanism of accelerating the rate of a chemical pathway. Finally, we speculate on the early evolutionary history of the cofactor and its ancient association with protein and RNA.

Keywords.

Enzyme thiamine diphosphate molecular evolution allostery structure and function catalysis cooperativity