Cellular and Molecular Life Sciences CMLS

, Volume 63, Issue 24, pp 2992–3017

Genetically engineered avidins and streptavidins

  • O. H. Laitinen
  • V. P. Hytönen
  • H. R. Nordlund
  • M. S. Kulomaa
Review

DOI: 10.1007/s00018-006-6288-z

Cite this article as:
Laitinen, O.H., Hytönen, V.P., Nordlund, H.R. et al. Cell. Mol. Life Sci. (2006) 63: 2992. doi:10.1007/s00018-006-6288-z

Abstract.

Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques to diagnostics, and from targeted drug delivery to nanotechnology. (Strept)avidin-biotin technology relies on the extremely tight and specific affinity between (strept)avidin and biotin (dissociation constant, Kd≈10−14–10−16 M). (Strept)avidins are also exceptionally stable proteins. To study their ligand binding and stability characteristics, the two proteins have been extensively modified both chemically and genetically. There are excellent accounts of this technology and chemically modified (strept)avidins, but no comprehensive reviews exist concerning genetically engineered (strept)avidins. To fill this gap, we here go through the genetically engineered (strept)avidins, summarizing how these constructs were designed and how they have improved our understanding of the structural and functional characteristics of these proteins, and the benefits they have provided for (strept)avidin-biotin technology.

Keywords.

Affinityavidinbionanotechnologybiotinmutagenesisprotein engineeringstreptavidin

Copyright information

© Birkhäuser Verlag, Basel 2006

Authors and Affiliations

  • O. H. Laitinen
    • 1
  • V. P. Hytönen
    • 2
  • H. R. Nordlund
    • 3
  • M. S. Kulomaa
    • 3
  1. 1.A. I. Virtanen Institute, Department of Molecular MedicineUniversity of KuopioKuopioFinland
  2. 2.Department of MaterialsETH ZürichZürichSwitzerland
  3. 3.Institute of Medical TechnologyUniversity of TampereBiokatu 6Finland