Cellular and Molecular Life Sciences CMLS

, Volume 63, Issue 21, pp 2546–2553

EP2 receptor stimulation promotes calcium responses in astrocytes via activation of the adenylyl cyclase pathway

Authors

  • A. Di Cesare
    • San Raffaele Scientific Institute
    • Vita-Salute San Raffaele University
  • P. Del Piccolo
    • San Raffaele Scientific Institute
    • San Raffaele Scientific Institute
    • IIT Research Unit of Molecular Neuroscience, DibitSan Raffaele Scientific Institute
    • San Raffaele Scientific Institute
    • Vita-Salute San Raffaele University
    • IIT Research Unit of Molecular Neuroscience, DibitSan Raffaele Scientific Institute
Research Article

DOI: 10.1007/s00018-006-6262-9

Cite this article as:
Di Cesare, A., Del Piccolo, P., Zacchetti, D. et al. Cell. Mol. Life Sci. (2006) 63: 2546. doi:10.1007/s00018-006-6262-9

Abstract.

Astrocytes are a heterogeneous population of cells that are endowed with a great variety of receptors for neurotransmitters and neuromodulators. Recently prostaglandin E2 has attracted great interest since it is not only released by astrocytes but also activates receptors coupled to either phospholipase C or adenylyl cyclase. We report that EP2 receptor stimulation triggers cAMP production but also causes release of Ca2+ from intracellular stores. This effect is shared by other receptors similarly coupled to adenylyl cyclase and elicited by direct stimulation of the enzyme or application of cAMP analogues. However, the stimulation of the Ca2+ response by cAMP is not mediated by protein kinase A, since a specific antagonist of this kinase had no effect. Such a cross-talk between cAMP and Ca2+ was not observed in all astrocytes. It might therefore reflect a specific resource of either a subpopulation or astrocytes in a specific functional state.

Keywords.

Prostaglandin E2butaprostsulprostoneisoproterenolforskolincAMP

Copyright information

© Birkhäuser Verlag, Basel 2006