Cellular and Molecular Life Sciences CMLS

, Volume 63, Issue 14, pp 1686–1699

Farnesylation of Pex19p is not essential for peroxisome biogenesis in yeast and mammalian cells

  • I. M. K. Vastiau
  • E. A. Anthonio
  • M. Brams
  • C. Brees
  • S. G. Young
  • S. Van de Velde
  • R. J. A. Wanders
  • G. P. Mannaerts
  • M. Baes
  • P. P. Van Veldhoven
  • M. Fransen
Research Article

DOI: 10.1007/s00018-006-6110-y

Cite this article as:
Vastiau, I.M.K., Anthonio, E.A., Brams, M. et al. Cell. Mol. Life Sci. (2006) 63: 1686. doi:10.1007/s00018-006-6110-y

Abstract.

Pex19p exhibits a broad binding specificity for peroxisomal membrane proteins (PMPs), and is essential for the formation of functional peroxisomal membranes. Pex19p orthologues contain a C-terminal CAAX motif common to prenylated proteins. In addition, Saccharomyces cerevisiae and Chinese hamster Pex19p are at least partially farnesylated in vivo. Whether farnesylation of Pex19p plays an essential or merely ancillary role in peroxisome biogenesis is currently not clear. Here, we show that (i) nonfarnesylated and farnesylated human Pex19p display a similar affinity towards a select set of PMPs, (ii) a variant of Pex19p lacking a functional farnesylation motif is able to restore peroxisome biogenesis in Pex19p-deficient cells, and (iii) peroxisome protein import is not affected in yeast and mammalian cells defective in one of the enzymes involved in the farnesylation pathway. Summarized, these observations indicate that the CAAX box-mediated processing steps of Pex19p are dispensable for peroxisome biogenesis in yeast and mammalian cells.

Keywords.

Peroxisomesbiogenesisperoxinsprotein importPex19pfarnesylation

Copyright information

© Birkhäuser Verlag, Basel 2006

Authors and Affiliations

  • I. M. K. Vastiau
    • 1
  • E. A. Anthonio
    • 1
  • M. Brams
    • 1
  • C. Brees
    • 1
  • S. G. Young
    • 2
  • S. Van de Velde
    • 3
    • 4
  • R. J. A. Wanders
    • 5
  • G. P. Mannaerts
    • 1
  • M. Baes
    • 6
  • P. P. Van Veldhoven
    • 1
  • M. Fransen
    • 1
  1. 1.Laboratorium voor Farmacologie, Departement Moleculaire Celbiologie, Faculteit GeneeskundeKatholieke Universiteit LeuvenLeuvenBelgium
  2. 2.Department of Medicine, Division of CardiologyUniversity of CaliforniaLos AngelesUSA
  3. 3.Laboratorium voor Moleculaire Celbiologie, Departement Biologie, Faculteit WetenschappenKatholieke Universiteit LeuvenLeuvenBelgium
  4. 4.Departement Moleculaire MicrobiologieVlaams Interuniversitair Instituut voor BiotechnologieLeuvenBelgium
  5. 5.Laboratorium Genetisch Metabole Ziekten, Departement voor PediatrieAcademisch Medisch Centrum,Universiteit van AmsterdamAmsterdamThe Netherlands
  6. 6.Laboratorium voor Klinische Chemie, Faculteit Farmaceutische WetenschappenKatholieke Universiteit LeuvenLeuvenBelgium