Cellular and Molecular Life Sciences CMLS

, Volume 62, Issue 9, pp 1025–1037

Modeling the MHC class I pathway by combining predictions of proteasomal cleavage,TAP transport and MHC class I binding

Authors

  • S. Tenzer
    • Institute of ImmunologyJohannes Gutenberg University
  • B. Peters
    • Institute for Biochemistry, University Medical School-ChariteHumboldt University
    • La Jolla Institute for Allergy and Immunology
  • S. Bulik
    • Institute for Biochemistry, University Medical School-ChariteHumboldt University
  • O. Schoor
    • Institute for Cell Biology, Department of ImmunologyUniversity of Tübingen
  • C. Lemmel
    • Institute for Cell Biology, Department of ImmunologyUniversity of Tübingen
  • M. M. Schatz
    • Institute of ImmunologyJohannes Gutenberg University
  • P.-M. Kloetzel
    • Institute for Biochemistry, University Medical School-ChariteHumboldt University
  • H.-G. Rammensee
    • Institute for Cell Biology, Department of ImmunologyUniversity of Tübingen
    • Institute of ImmunologyJohannes Gutenberg University
  • H.-G. Holzhütter
    • Institute for Biochemistry, University Medical School-ChariteHumboldt University
Research Article

DOI: 10.1007/s00018-005-4528-2

Cite this article as:
Tenzer, S., Peters, B., Bulik, S. et al. CMLS, Cell. Mol. Life Sci. (2005) 62: 1025. doi:10.1007/s00018-005-4528-2
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Abstract.

Epitopes presented by major histocompatibility complex (MHC) class I molecules are selected by a multi-step process. Here we present the first computational prediction of this process based on in vitro experiments characterizing proteasomal cleavage, transport by the transporter associated with antigen processing (TAP) and MHC class I binding. Our novel prediction method for proteasomal cleavages outperforms existing methods when tested on in vitro cleavage data. The analysis of our predictions for a new dataset consisting of 390 endogenously processed MHC class I ligands from cells with known proteasome composition shows that the immunological advantage of switching from constitutive to immunoproteasomes is mainly to suppress the creation of peptides in the cytosol that TAP cannot transport. Furthermore, we show that proteasomes are unlikely to generate MHC class I ligands with a C-terminal lysine residue, suggesting processing of these ligands by a different protease that may be tripeptidyl-peptidase II (TPPII).

Key words.

ProteasomeTAPMHCepitope predictionantigen processing

Copyright information

© Birkhäuser Verlag, Basel 2005