Cellular and Molecular Life Sciences CMLS

, Volume 61, Issue 18, pp 2405–2417

Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines

  • P. Wang
  • E. Mariman
  • J. Keijer
  • F. Bouwman
  • J.-P. Noben
  • J. Robben
  • J. Renes
Research Article

DOI: 10.1007/s00018-004-4256-z

Cite this article as:
Wang, P., Mariman, E., Keijer, J. et al. CMLS, Cell. Mol. Life Sci. (2004) 61: 2405. doi:10.1007/s00018-004-4256-z

Abstract

Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20°C treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation.

3T3-L1 adipocytesecretionproteomicsextracellular matrixgrowth regulation

Copyright information

© Birkhäuser-Verlag Basel 2004

Authors and Affiliations

  • P. Wang
    • 1
  • E. Mariman
    • 1
  • J. Keijer
    • 2
  • F. Bouwman
    • 1
  • J.-P. Noben
    • 3
  • J. Robben
    • 3
  • J. Renes
    • 1
  1. 1.Maastricht Proteomics Center, The Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Department of Human BiologyMaastricht UniversityMaastrichtThe Netherlands
  2. 2.RIKILT-Institute of Food SafetyWageningenThe Netherlands
  3. 3.Biomedical Research Institute, Limburgs Universitair Centrum and School of Life SciencesTransnational University LimburgDiepenbeekBelgium