Cellular and Molecular Life Sciences CMLS

, Volume 61, Issue 17, pp 2264–2273

Hcc-1 is a novel component of the nuclear matrix with growth inhibitory function

Research Article

DOI: 10.1007/s00018-004-4205-x

Cite this article as:
Leaw, C.L., Ren, E.C. & Choong, M.L. CMLS, Cell. Mol. Life Sci. (2004) 61: 2264. doi:10.1007/s00018-004-4205-x


Hcc-1 is a novel nuclear protein containing the SAF-box DNA-binding domain. It binds to both double-stranded and single-stranded DNA with higher affinity for the single-stranded form. In addition, it also binds specifically to scaffold/matrix attachment region DNA. These nucleic acid-binding characteristics suggest a potential function for Hcc-1 as a component of the heterogeneous ribonucleoprotein complex. Using a yeast two-hybrid screen, two DEAD-box RNA helicases, BAT1 and DDX39, were identified as proteins that interact with Hcc-1. Interactions with these RNA helicases suggested a role for Hcc-1 in nucleic acid biogenesis. Expression of Hcc-1 in the HEK293 cell line resulted in a slower growth rate compared to controls (p = 0.0173) and an accumulation of cells at the G2/M phase (p = 0.0276 compared to control HEK293 cells). Taken together, these results suggest a role for Hcc-1 in growth regulation and nucleic acids metabolism.

DEAD-box familyheterogeneous ribonucleoproteinnuclear matrix proteinSAF-boxscaffold attachment region DNA

Copyright information

© Birkhäuser-Verlag Basel 2004

Authors and Affiliations

  1. 1.Bioprocessing Technology Institute, Agency for ScienceTechnology and Research (A*STAR)Singapore
  2. 2.Department of MicrobiologyNational University of SingaporeSingapore
  3. 3.W.H.O., Collaborating Centre for Research and Training in ImmunologyNational University of Singapore, Block MD4SingaporeSingapore