Cellular and Molecular Life Sciences CMLS

, Volume 61, Issue 11, pp 1341–1353

Annotating proteins from endoplasmic reticulum and Golgi apparatus in eukaryotic proteomes

Research Article

DOI: 10.1007/s00018-004-4005-3

Cite this article as:
Wrzeszczynski, K.O. & Rost, B. CMLS, Cell. Mol. Life Sci. (2004) 61: 1341. doi:10.1007/s00018-004-4005-3

Abstract

The sub-cellular localization of a native protein constitutes one coarse-grained aspect of its function. Transport between compartments is often regulated through short sequence motifs. Here, we analyzed experimentally characterized endoplasmic reticulum (ER)/ Golgi retrieval motifs and investigated the accuracy of homology-transfer. Only the C-terminal ER retrieval motifs KDEL, HDEL and AIAKE were sufficiently specific. However, even unspecific motifs may help, provided we know the probability for localization given the motif. We provided such estimates. We also rigorously estimated the accuracy and coverage for inferring ER and Golgi localization through homology-transfer by sequence similarity. In entire proteomes, we could thereby annotate 3304 ER (3182 membrane) and 1853 Golgi (759 membrane) proteins. We identified another putative 5157 globular and 3941 membrane ER or Golgi proteins. Each experimental annotation yielded, on average, one to three high-accuracy and five to six low-accuracy homology-transfers in the six proteomes. These numbers will increase with each new experimental annotation.

Endoplasmic reticulumGolgi apparatusgenome sequence analysissub-cellular localizationprotein sequence motifs

Copyright information

© Birkhäuser-Verlag Basel 2004

Authors and Affiliations

  1. 1.CUBIC, Department of Biochemistry and Molecular BiophysicsColumbia UniversityNew YorkUSA
  2. 2.Integrated Program in Cellular, Molecular and Biophysical StudiesColumbia UniversityNew YorkUSA
  3. 3.Center for Computational Biology and Bioinformatics (C2B2), Russ Berrie PavilionColumbia UniversityNew YorkUSA
  4. 4.North East Structural Genomics Consortium (NESG), Department of Biochemistry and Molecular BiophysicsColumbia UniversityNew YorkUSA