Cellular and Molecular Life Sciences CMLS

, Volume 61, Issue 4, pp 502–509

High affinity recognition of a Phytophthora protein by Arabidopsis via an RGD motif

  • V. Senchou
  • R. Weide
  • A. Carrasco
  • H. Bouyssou
  • R. Pont-Lezica
  • F. Govers
  • H. Canut
Research Article

DOI: 10.1007/s00018-003-3394-z

Cite this article as:
Senchou, V., Weide, R., Carrasco, A. et al. CMLS, Cell. Mol. Life Sci. (2004) 61: 502. doi:10.1007/s00018-003-3394-z

Abstract

The RGD tripeptide sequence, a cell adhesion motif present in several extracellular matrix proteins of mammalians, is involved in numerous plant processes. In plant-pathogen interactions, the RGD motif is believed to reduce plant defence responses by disrupting adhesions between the cell wall and plasma membrane. Photoaffinity cross-linking of [125I]-azido-RGD heptapeptide in the presence of purified plasma membrane vesicles of Arabidopsis thaliana led to label incorporation into a single protein with an apparent molecular mass of 80 kDa. Incorporation could be prevented by excess RGD peptides, but also by the IPI-O protein, an RGD-containing protein secreted by the oomycete plant pathogen Phytophthora infestans. Hydrophobic cluster analysis revealed that the RGD motif of IPI-O (positions 53–56) is readily accessible for interactions. Single amino acid mutations in the RGD motif in IPI-O (of Asp56 into Glu or Ala) resulted in the loss of protection of the 80-kDa protein from labelling. Thus, the interaction between the two proteins is mediated through RGD recognition and the 80-kDa RGD-binding protein has the characteristics of a receptor for IPI-O. The IPI-O protein also disrupted cell wall-plasma membrane adhesions in plasmolysed A. thaliana cells, whereas IPI-O proteins mutated in the RGD motif (D56A and D56E) did not.

Cell signalling RGD motif plasma membrane cell wall Phytophthora infestans Arabidopsis thaliana 

Copyright information

© Birkhäuser-Verlag Basel 2004

Authors and Affiliations

  • V. Senchou
    • 1
    • 2
  • R. Weide
    • 2
  • A. Carrasco
    • 1
  • H. Bouyssou
    • 1
  • R. Pont-Lezica
    • 1
  • F. Govers
    • 2
  • H. Canut
    • 1
  1. 1.Surfaces Cellulaires et Signalisation chez les VégétauxUMR 5546 CNRS-Université Paul SabatierCastanet Tolosan cedexFrance
  2. 2.Laboratory of PhytopathologyWageningen UniversityWageningenThe Netherlands