Cellular and Molecular Life Sciences CMLS

, Volume 60, Issue 7, pp 1477–1488

Thiamine triphosphate and thiamine triphosphatase activities: from bacteria to mammals

  • A. F. Makarchikov
  • B. Lakaye
  • I. E. Gulyai
  • J. Czerniecki
  • B. Coumans
  • P. Wins
  • T. Grisar
  • L. Bettendorff
Research Article

DOI: 10.1007/s00018-003-3098-4

Cite this article as:
Makarchikov, A.F., Lakaye, B., Gulyai, I.E. et al. CMLS, Cell. Mol. Life Sci. (2003) 60: 1477. doi:10.1007/s00018-003-3098-4

Abstract

In most organisms, the main form of thiamine is the coenzyme thiamine diphosphate. Thiamine triphosphate (ThTP) is also found in low amounts in most vertebrate tissues and can phosphorylate certain proteins. Here we show that ThTP exists not only in vertebrates but is present in bacteria, fungi, plants and invertebrates. Unexpectedly, we found that in Escherichia coli as well as in Arabidopsis thaliana, ThTP was synthesized only under particular circumstances such as hypoxia (E. coli) or withering (A. thaliana). In mammalian tissues, ThTP concentrations are regulated by a specific thiamine triphosphatase that we have recently characterized. This enzyme was found only in mammals. In other organisms, ThTP can be hydrolyzed by unspecific phosphohydrolases. The occurrence of ThTP from prokaryotes to mammals suggests that it may have a basic role in cell metabolism or cell signaling. A decreased content may contribute to the symptoms observed during thiamine deficiency.

Thiamine triphosphateThiamine triphosphatasebacteriaplantsanimalsstressadenylyl cyclasevitamin B1

Copyright information

© Birkhäuser-Verlag Basel 2003

Authors and Affiliations

  • A. F. Makarchikov
    • 1
    • 2
  • B. Lakaye
    • 1
  • I. E. Gulyai
    • 2
  • J. Czerniecki
    • 1
    • 3
  • B. Coumans
    • 1
  • P. Wins
    • 1
  • T. Grisar
    • 1
  • L. Bettendorff
    • 1
  1. 1.Center for Cellular and Molecular NeurobiologyUniversity of LiègeLiègeBelgium
  2. 2.Laboratory of Enzymology, Institute of BiochemistryNational Academy of Sciences of BelarusGrodnoBelarus
  3. 3.Institute of BiologyUniversity of BialystokBialystokPoland