Effect of lipopolysaccharide on D-fructose transport across rabbit jejunum
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- García-Herrera, J., Abad, B. & Rodríguez-Yoldi, M. Inflamm. res. (2003) 52: 177. doi:10.1007/s000110300069
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Objective: To investigate alterations in the transport of D-fructose across the rabbit jejunum when the gut is exposed in vitro to lipopolysaccharide (LPS), an endotoxin causative agent of sepsis. Materials and methods: D-fructose intestinal transport was assesed employing three techniques: sugar uptake measurements in rings of everted jejunum (μmol/D-fructose/ml cell water), transepithelial flux measurements in Ussing-type chambers (μmol D-fructose/cm2/h) and transport assays in preparation of brush border membrane vesicles (pmoles D-fructose/mg protein). Samples were taken from the bathing solution and from the extracts of the tissue for radioactivity counting. Results: Adding LPS (3 μg/ml) to tissue decreased the uptake and mucosal to serosal flux of 5 mM D-fructose across the enterocyte. LPS did not modify sugar uptake across brush border membrane vesicles. The inhibitory effect of LPS was suppressed by W-13 (5 × 10–6 M), a Ca-calmodulin antagonist, and staurosporine (10–7 and 10–6 M) and GF-109203X (10–6 M) a nonselective and selective protein kinase C (PKC) inhibitor respectively. Tumor Necrosis Factor (TNF-α), an immunoregulatory cytokine involved in septic responses occurring during bacterial infection at concentrations 3 × 10–4 to 3 μg/ml, did not affect the sugar transport. Conclusions: LPS can inhibit the intestinal uptake of D-fructose across the rabbit jejunum in vitro by intracellular processes related to PKC and calmodulin protein.