Inflammation Research

, Volume 48, Issue 10, pp 539–543

Differential IL-10 receptor gene expression in acute versus chronic atopic eczema. Modulation by immunosuppressive drugs and cytokines in normal cultured keratinocytes

  • A. Müschen
  • A. Mirmohammadsadegh
  • B. Jarzebska-Deussen
  • H. F. Abts
  • T. Ruzicka
  • G. Michel

DOI: 10.1007/s000110050500

Cite this article as:
Müschen, A., Mirmohammadsadegh, A., Jarzebska-Deussen, B. et al. Inflamm. res. (1999) 48: 539. doi:10.1007/s000110050500

Abstract.

Objective and Design: The effects of the anticytokine interleukin 10 (IL-10) are mediated by specific receptors. In this study we examined the role of the IL-10 receptor (IL-10R) in the pathophysiology of atopic eczema. ¶Materials and Methods: For this purpose we analyzed the expression of IL-10R in the skin of patients with acute and chronic atopic eczema in comparison to the expression in healthy individuals using in situ binding experiments with fluorescently labeled IL-10 and semiquantitative reverse transcriptase-PCR specific for IL-10R1. In addition, we studied the influence of the Th2-associated cytokine interleukin-4 (IL-4), the Th1-associated gamma-interferon (IFN-γ), the immunosuppressive drug FK506, the H1-antagonist loratadine and UVA irradiation on the expression of IL-10R1 in cultured normal human keratinocytes. ¶Results: We found that IL-10 receptor mRNA and protein are strongly downregulated in acute phase atopic lesions. Furthermore we could show that IL-4, IFN-γ, FK506, loratadine and UVA enhance the mRNA levels of the IL-10R1 in vitro in normal cultured keratinocytes. We could also demonstrate restored IL-10R1 mRNA levels in lesional atopic skin of a patient after UVA1 therapy. ¶Conclusions: Our results demonstrate for the first time that IL-10 receptors may have a role in the pathogenesis of atopic eczema and its upregulation by FK506 and UVA could explain the therapeutic efficacy of these agents.

Key words: Atopic eczema — IL-10 receptor — FK506 — Loratadine — Ultraviolet irradiation

Copyright information

© Birkhäuser Verlag, 1999

Authors and Affiliations

  • A. Müschen
    • 1
  • A. Mirmohammadsadegh
    • 1
  • B. Jarzebska-Deussen
    • 1
  • H. F. Abts
    • 1
  • T. Ruzicka
    • 1
  • G. Michel
    • 1
  1. 1.Department of Dermatology, Heinrich-Heine-University Düsseldorf, Moorenstr. 5, D-40225 Düsseldorf, Germany, Fax +492118117316, e-mail: michelg@rz.uni-duesseldorf.de DE