Article

Inflammation Research

, Volume 48, Issue 4, pp 199-204

First online:

Osteogenic protein-1 (OP-1) blocks cartilage damage caused by fibronectin fragments and promotes repair by enhancing proteoglycan synthesis

  • H. E. KoeppAffiliated withDepartment of Orthopaedic Surgery, University of Ulm, Oberer Eselsberg 45, D-89081 Ulm/Donau, Germany
  • , K. T. SampathAffiliated withCreative BioMolecules, Hopkinton, MA, USA
  • , K. E. KuettnerAffiliated withDepartment of Biochemistry, Rush Medical College at Rush-Presbyterian-St. Luke's Medical Center, 1653 West Congress Parkway, Chicago, IL 60612-3864, USA, Fax +1 312 9423053, e-mail: ghomandb@rush.edu
  • , G. A. HomandbergAffiliated withDepartment of Biochemistry, Rush Medical College at Rush-Presbyterian-St. Luke's Medical Center, 1653 West Congress Parkway, Chicago, IL 60612-3864, USA, Fax +1 312 9423053, e-mail: ghomandb@rush.edu

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Abstract

Objective and Design: The abilities of osteogenic protein-1 (OP-1) and TGF-β1 to affect cartilage damage caused by fibronectin fragments (Fn-fs) that are known to greatly enhance cartilage proteoglycan (PG) degradation were compared.¶Material: Articular cartilage was obtained from 18 month old bovines.¶Treatment: To test blocking of damage, cartilage was cultured with or without OP-1 or TGF-β in the presence of 100 nM Fn-fs. To test restoration of PG, cartilage was first cultured with Fn-fs and the cartilage then treated with factors.¶Methods: Cartilage PG content was measured in papain digests using the dimethylmethylene blue assay. PG synthesis was measured by incorporation of 35S labeled sulfate.¶Results: OP-1 blocked damage and restored PG in damaged cartilage, apparently due to enhanced PG synthesis. However, TGF-β1 alone decreased PG content.¶Conclusions: These results clearly demonstrate differences between OP-1 and TGF-β1, both members of the TGF-β superfamily and illustrate the efficacy of OP-1 in blocking Fn-f mediated damage.

Key words: OP-1 — TGF-β— Fibronectin fragments — Cartilage — Cartilage repair