Inflammation Research

, Volume 48, Issue 3, pp 139–148

Suppression of carrageenan-induced inflammation in vivo by an extract of the Chinese herbal remedy Tripterygium wilfordii Hook F

  • X. Tao
  • L. Ma
  • Y. Mao
  • P. E. Lipsky
Article

DOI: 10.1007/s000110050437

Cite this article as:
Tao, X., Ma, L., Mao, Y. et al. Inflamm. res. (1999) 48: 139. doi:10.1007/s000110050437

Abstract.

Objective and Design: The current studies investigated the effect of subacute administration of an ethyl acetate extract of the Chinese herbal remedy, Tripterygium wilfordii Hook F (TWHF) on the inflammatory response induced by carrageenan in the rat.¶Materials and Subjects: F344 rats were randomly divided into 3 groups of seven animals.¶Treatment: The rats were treated orally for 5 days with either vehicle or an ethyl acetate extract of TWHF at a dose of 270 mg/kg, equivalent to 1/5 of the dose causing death in 50% of the animals.¶Methods: Air pouches were induced subcutaneously on the backs of rats and injected with carrageenan on the last day of treatment. Sixteen hours after carrageenan challenge, the air pouches were removed and analyzed. Student's t-test was employed for statistical analysis.¶Results: The number of exudate cells and the concentration of PGE2, nitrite and TNF-α in the exudate obtained from TWHF-treated animals were significantly reduced (by 69%, 78%, 57% and 77%, respectively) compared to that from vehicle-treated animals. mRNA for cyclooxygenase-2 was markedly suppressed in the air pouch lining tissue of TWHF treated rats (p < 0.001). In contrast, PGE2 content of the kidney and stomach and the production of PGE2, nitrite and TNF-α by spleen cells was not affected by treatment with TWHF.¶Conclusions: These results indicate that TWHF exerts a significant anti-inflammatory activity in vivo.

Key words: Tripterygium wilfordii Hook F — Anti-inflammatory agent — Inflammatory mediators — Cyclooxygenase-2 

Copyright information

© Birkhäuser Verlag, Basel, 1999

Authors and Affiliations

  • X. Tao
    • 1
  • L. Ma
    • 1
  • Y. Mao
    • 1
  • P. E. Lipsky
    • 1
  1. 1.Harold C. Simmons Arthritis Research Center and Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75235-8884, USA, Fax +1 214 648 7995, e-mail: peter.lipsky@email.swmed.eduUSA

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