Inflammation Research

, Volume 47, Issue 12, pp 482–487

Expression of cyclooxygenase isoforms in the rat spinal cord and their regulation during adjuvant-induced arthritis

  • F. Beiche
  • K. Brune
  • G. Geisslinger
  • M. Goppelt-Struebe

DOI: 10.1007/s000110050362

Cite this article as:
Beiche, F., Brune, K., Geisslinger, G. et al. Inflamm. res. (1998) 47: 482. doi:10.1007/s000110050362

Abstract.

Objective and Design: Spinal regulation of cyclooxygenase (COX) isoforms was investigated in the animal model of peripheral inflammation induced by injection of complete Freund's-type adjuvant (CFA) in the rat hindpaw.¶Subjects and Treatment: Peripheral inflammation was induced by intraplantar injection of CFA in one hind footpad of male Sprague Dawley rats (n = 3 per time point).¶Methods: Spinal cord was removed after different times (3 h to 22 d). mRNA and protein were isolated and analyzed by comparative reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively.¶Results: Under the acute inflammatory stimulus 6 h after CFA application, RT-PCR revealed a twofold increase in COX-2 mRNA that reached baseline again at day 3. This transient increase occurred in the lumbar spinal cord, but changes in COX-2 mRNA expression were also registered in RNA preparations from cervical sections, spinal COX-2 induction thus not being a spatially confirmed phenomenon. Western blot analysis of spinal membrane preparations reflected the transient COX-2 mRNA induction at protein levels. During the chronic phase of arthritis at day 22, COX-2 levels were again raised significantly (1.6 fold) over baseline. Spinal levels of COX-1 were not altered at any time point of the peripheral inflammation.¶Conclusion: These data imply a regulatory role for COX-2 but not COX-1 in the spinal modulation under acute and chronic peripheral inflammation.

Key words: Cyclooxygenase — Prostaglandin G/H synthase — Spinal cord — Inflammation — RT-PCR

Copyright information

© Birkhäuser Verlag, Basel, 1998

Authors and Affiliations

  • F. Beiche
    • 1
  • K. Brune
    • 2
  • G. Geisslinger
    • 2
  • M. Goppelt-Struebe
    • 1
  1. 1.Medizinische Klinik IV, Universität Erlangen-Nürnberg, Loschgestr. 8, D-91054 Erlangen, Germany, Fax +49 9131 8539202, e-mail: mfm421@rzmail.uni-erlangen.deDE
  2. 2.Institut für Experimentelle und Klinische Pharmakologie und Toxikologie, Universität Erlangen-Nürnberg, Universitätsstr. 22, D-91054 Erlangen, GermanyDE