Inflammation Research

, Volume 47, Issue 11, pp 421–427

Anti-inflammatory activity of myricetin-3-O-β-D-glucuronide and related compounds

  • A. Hiermann
  • H. W. Schramm
  • S. Laufer
Article

DOI: 10.1007/s000110050355

Cite this article as:
Hiermann, A., Schramm, H. & Laufer, S. Inflamm. res. (1998) 47: 421. doi:10.1007/s000110050355

Abstract.

Objective and Design: The anti-inflammatory effect of myricetinglucuronide (MGL) was investigated and structurally-related compounds were compared to examine the structure/activity-relationship in carrageenan-induced rat paw edema.¶Materials and Subjects: In vitro studies were performed using rat basophilic leukemia (RBL-1) cells, human polymorphonuclear leukocytes (PMNL), COX-1 from ram seminal vesicle, COX-2 from sheep placenta and human venous blood. For the in vivo tests male Wistar rats were used, for the ex vivo test perfused rabbit ears.¶Treatment: 1–300 μg/kg MGL or myricetinmethylglucuronate and 0.1–5 mg/kg other related compounds administered p.o. (carrageenan edema). 5, 50 and 150 μg/kg MGL p.o. for 14 days (Freund's adjuvant arthritis), 5 and 50 μg/kg p.o. for 6 days (ulceration).¶Methods: Anti-inflammatory effects were measured in carrageenan edema and in adjuvant arthritis. Incidence of gastric lesions was tested in an ulcerogenicity model in vivo. Influence on COX was determined in the perfused rabbit ear, in PMNL and in a test assay using COX-1 and COX-2. 5-LOX activity was studied using PMNL and RBL-1. The influence on platelet aggregation was evaluated measuring light transmission.¶Results: MGL exerted a marked and dose-dependent anti-inflammatory effect in acute (carrageenan edema, ED5 15 μg/kg, indomethacin ED50 10 mg/kg) and chronic (adjuvant arthritis, inhibition at 150 μg/kg 18.1% left paw, 20.6% right paw, indomethacin 3 mg/kg 18.0% and 19.4%)) models of inflammation. In the perfused rabbit ear 1 μg MGL inhibited the release of PGI2, PGD2 and PGE2 to the same extent as 1 μg indomethacin. The inhibition of COX-1 in the intact cell system was IC50 = 0.5 μM, that of indomethacin 0.0038 μM. In the isolated enzyme preparations of COX-1 and COX-2 the IC50 was 10 μM and 8 μM, that of indomethacin 9.2 mM and 2.4 μM. In the RBL-1 and PMNL test assay the inhibition of 5-LOX was 0.1 μM and 2.2 μM. An orally administered dose of 50 μg/kg/day induced no gastric ulcers in rats treated for 6 days. The investigations on carrageenan edema showed a close relationship between the structure of MGL and the anti-inflammatory effect.¶Conclusions: MGL is a COX-1, COX-2 and 5-LOX inhibitor. In view of the moderate in vitro activity and the very potent in vivo activity an additive mechanism must be involved. Small changes in the molecular structure lead to the loss or reduction of the anti-inflammatory activity.

Key words: Myricetin-3-O-β-D-glucuronide — Related compounds — Anti-inflammatory effects

Copyright information

© Birkhäuser Verlag, Basel, 1998

Authors and Affiliations

  • A. Hiermann
    • 1
  • H. W. Schramm
    • 2
  • S. Laufer
    • 3
  1. 1.Institute of Pharmacognosy, University of Graz, Universitätsplatz 4, A-8010 Graz, Austria, e-mail: alois.hiermann@kfunigraz.ac.atAT
  2. 2.Institute of Pharmaceutical Chemistry, University of Graz, Universitätsplatz 1, A-8010 Graz, AustriaAT
  3. 3.Merckle GmbH, Graf Arco Str. 3, D-89079 Ulm, GermanyDE