Inflammation Research

, Volume 65, Issue 2, pp 133–142

Characterization of changes in plasma and tissue oxylipin levels in LPS and CLP induced murine sepsis

  • Ina Willenberg
  • Katharina Rund
  • Song Rong
  • Nelli Shushakova
  • Faikah Gueler
  • Nils Helge Schebb
Original Research Paper

DOI: 10.1007/s00011-015-0897-7

Cite this article as:
Willenberg, I., Rund, K., Rong, S. et al. Inflamm. Res. (2016) 65: 133. doi:10.1007/s00011-015-0897-7

Abstract

Objective

The present study aimed to comprehensively investigate the changes in oxylipins during murine sepsis induced by lipopolysaccharide (LPS) or cecal ligation and puncture (CLP).

Methods

Twenty-four hours after induction of sepsis in male C57BL/6 mice by LPS or CLP, plasma and liver, lung, kidney and heart tissues were sampled. Oxylipin levels in plasma and tissue were quantified by means of LC–MS. Moreover, clinical chemistry parameters were determined in plasma and interleukin levels (MCP-1 and IL-6) were determined in kidney and liver.

Results

Elevation of liver function plasma parameters at 24 h revealed that both models were successful in the induction of sepsis. LPS induced sepsis resulted in a dramatic increase of plasma PGE2 (2,100 % change in comparison to control) and other cyclooxygenase metabolites, whereas this effect was less pronounced in CLP induced sepsis (97 % increase of PGE2). Plasma epoxy-fatty acids (FAs) and hydroxy-FAs and most of the dihydroxy-FAs were elevated in both models of sepsis. Changes of tissue oxylipin concentrations were organ dependent. Only few changes were detected in the lung and liver tissue, epoxy-FAs were elevated in the kidney. In the heart tissue a trend towards lower levels of hydroxy-FAs and epoxy-FAs was observed.

Conclusion

Both murine models of sepsis are characterized by changes of oxylipins formed in all branches of the arachidonic acid (AA) cascade. The more pronounced effects in the LPS model make this model more suitable for the investigation of the AA cascade and its pharmacological modulation in sepsis.

Keywords

Sepsis Lipopolysaccharide (LPS) Cecal ligation and puncture Lipid mediators 

Supplementary material

11_2015_897_MOESM1_ESM.pdf (426 kb)
Supplementary Information (PDF 426 kb)

Copyright information

© Springer International Publishing 2015

Authors and Affiliations

  • Ina Willenberg
    • 1
  • Katharina Rund
    • 1
  • Song Rong
    • 2
    • 3
  • Nelli Shushakova
    • 2
    • 4
  • Faikah Gueler
    • 2
    • 4
  • Nils Helge Schebb
    • 1
    • 5
  1. 1.Institute for Food Toxicology and Analytical ChemistryUniversity of Veterinary Medicine Hannover30173Germany
  2. 2.Department of NephrologyHannover Medical SchoolHannoverGermany
  3. 3.The Transplantation Center of the Affiliated HospitalZunyi Medical CollegeZunyiChina
  4. 4.Phenos GmbHHannoverGermany
  5. 5.Institute of Food ChemistryUniversity of WuppertalWuppertalGermany