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The phenyl-thiophenyl propenone RK-I-123 reduces the levels of reactive oxygen species and suppresses the activation of NF-κB and AP-1 and IL-8 expression in Helicobacter pylori-infected gastric epithelial AGS cells

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Abstract

Objective

To investigate whether the phenyl-thiophenyl propenone RK-I-123 suppresses interleukin-8 (IL-8) expression and activation of mitogen-activated protein kinases (MAPKs) and transcription factors (nuclear factor-κB [NF-κB] and activator protein-1 [AP-1]) by reducing reactive oxygen species (ROS) levels in Helicobacter pylori-infected gastric epithelial cells.

Material

Helicobacter pylori in Korean isolates, human gastric epithelial AGS cells.

Treatment

AGS cells pretreated with or without RK-I-123 were cultured in the presence of H. pylori at a bacterium/cell ratio of 300:1.

Methods

Reactive oxygen species and IL-8 levels were determined by dichlorofluorescein fluorescence and enzyme-linked immunosorbent assay. The IL-8 mRNA expression was analyzed by the real-time reverse transcription-polymerase chain reaction (RT-PCR). The MAPK and IκBα levels were determined by western blotting. The activation of NF-κB and AP-1 was determined by the electrophoretic mobility shift assay.

Results

Helicobacter pylori induced an increase in ROS and IL-8 expression and activation of MAPKs and transcription factors (NF-κB and AP-1) together with the degradation of IκBα in AGS cells, all of which were inhibited by RK-I-123.

Conclusions

The RK-I-123 suppressed the H. pylori-induced IL-8 expression and activation of MAPKs, NF-κB, and AP-1 by reducing ROS levels in AGS cells. The RK-I-123 may be a potential candidate for the treatment of H. pylori-induced gastric inflammation.

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Acknowledgments

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0000811). H. Kim is grateful to the Brain Korea 21 Project of the College of Human Ecology at Yonsei University.

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Authors and Affiliations

  1. Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, Seoul, 120-749, Korea

    Sung Hee Jang, Soonok Cho & Hyeyoung Kim

  2. College of Pharmacy, Yeungnam University, Kyongsan, 712-749, Korea

    Eung-Seok Lee

  3. Department of Microbiology, Hanyang University College of Medicine, Seoul, 133-791, Korea

    Jung Mogg Kim

Authors
  1. Sung Hee Jang
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  2. Soonok Cho
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  3. Eung-Seok Lee
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  4. Jung Mogg Kim
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  5. Hyeyoung Kim
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Correspondence to Hyeyoung Kim.

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Responsible Editor: Mauro Teixeira.

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Jang, S.H., Cho, S., Lee, ES. et al. The phenyl-thiophenyl propenone RK-I-123 reduces the levels of reactive oxygen species and suppresses the activation of NF-κB and AP-1 and IL-8 expression in Helicobacter pylori-infected gastric epithelial AGS cells. Inflamm. Res. 62, 689–696 (2013). https://doi.org/10.1007/s00011-013-0621-4

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  • DOI: https://doi.org/10.1007/s00011-013-0621-4

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