Inflammation Research

, Volume 62, Issue 2, pp 213–218

The effects of various doses of bacterial lipopolysaccharide on the expression of CD63 and the release of histamine by basophils of atopic and non-atopic patients

  • E. Gyimesi
  • F. Gönczi
  • M. Szilasi
  • G. Pál
  • S. Baráth
  • S. Sipka
Original Research Paper

DOI: 10.1007/s00011-012-0569-9

Cite this article as:
Gyimesi, E., Gönczi, F., Szilasi, M. et al. Inflamm. Res. (2013) 62: 213. doi:10.1007/s00011-012-0569-9
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Abstract

Objective

We tested the effect of various doses of bacterial lipopolysaccharide (LPS, endotoxin) on the expression of CD63 and the in vitro release of histamine by basophils stimulated with ragweed allergen in patients with or without ragweed and mite allergies.

Methods

The peripheral blood of 11 patients with ragweed allergy, 10 patients with mite allergy and 14 control patients was incubated with ragweed allergen extract following pretreatment with varying doses of LPS. The expression of CD63 in basophils was measured by flow cytometry, and the release of histamine was determined by ELISA.

Results

In the samples of patients with ragweed allergy that were exposed to specific allergen, only high doses of LPS significantly elevated the expression of CD63 (200 ng/ml; 1,000 EU/ml) and the release of histamine (2,000 ng/ml; 10,000 EU/ml). There was no effect of LPS in any other cases.

Conclusions

Bacterial LPS (endotoxin) concentrations higher than 200 ng/ml (1,000 EU/ml), which rarely occurs in nature, could only activate the basophils from atopic patients whilst in the presence of the specific allergen. Thus, the restoration of the urban, “microbe-poor” milieu with endotoxin (as LPS) can be a promising and harmless approach for allergy prevention.

Keywords

Bacterial lipopolysaccharide (endotoxin)CD63HistamineAtopic allergy

Copyright information

© Springer Basel 2012

Authors and Affiliations

  • E. Gyimesi
    • 1
  • F. Gönczi
    • 2
  • M. Szilasi
    • 3
  • G. Pál
    • 1
  • S. Baráth
    • 1
  • S. Sipka
    • 1
  1. 1.Division of Clinical Immunology, Department of Medicine, Medical and Health Science CentreUniversity of DebrecenDebrecenHungary
  2. 2.County HospitalDebrecenHungary
  3. 3.Department of Pulmonology, Medical and Health Sciences CenterUniversity of DebrecenDebrecenHungary