Inflammation Research

, 60:1061

Role of nitric oxide (NO) metabolism and inflammatory mediators in childhood obesity

  • András Hrabák
  • László Derzbach
  • Ildikó Csuka
  • Tamás Bajor
  • Anna Körner
Original Research Paper

DOI: 10.1007/s00011-011-0367-9

Cite this article as:
Hrabák, A., Derzbach, L., Csuka, I. et al. Inflamm. Res. (2011) 60: 1061. doi:10.1007/s00011-011-0367-9
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Abstract

Objective and design

The role of NO and adipocytokines in childhood obesity was studied, supposing that obesity provokes inflammation. Children were admitted to the pediatric clinic for a regular check up because of obesity.

Subjects

Obese (n = 79) and healthy (n = 12) children were selected and divided into subgroups according to their age, gender, glucose tolerance and nitric oxide synthase (NOS II) positivity.

Methods

Urine and blood nitrite plus nitrate, the expression of NOS II in white blood cells, serum adipocytokines and clinical characteristics were analyzed in each group. Significance was tested by unpaired two-tailed t test and by ANOVA.

Results

NOS II was only detected in the white blood cells of a subgroup (17/79) of obese children. Serum leptin and resistin concentrations were significantly higher, adiponectin was lower compared to healthy children. Significant correlations were observed between serum adiponectin and resistin levels (reciprocal, R2 = 0.4), and between body mass index and serum leptin levels.

Conclusions

NOS II expression in white blood cells was observed in a minority of patients. Low-grade inflammation in obese children was suggested by the increased resistin levels, particularly in NOS II-positive patients. Correlation between different adipocytokines was restricted for a few subgroups.

Keywords

Adiponectin Leptin Resistin Nitric oxide Obese children 

Abbreviations

Akt/PKB

Protein kinase B

AP-1

Activator protein 1

BMI

Body mass index

CRP

C-reactive protein

EDTA

Ethylene-diamine tetraacetate

ELISA

Enzyme-linked immunosorbent assay

Erk

Extracellular signal-regulated kinase

HDL

High density lipoprotein

HEPES

N-(2-hydroxyethyl) piperazinyl-N′-(2-ethane sulfonic acid)

HOMA

Homeostasis Model Assessment

IGT

Impaired glucose tolerance

IFG

Impaired fasting glucose

IKK

Inhibitor of κB kinase

IL-6

Interleukin-6

JNK

C-jun terminal kinase

MAPK

Mitogen-activated protein kinase

NF-κB

Nuclear factor κB

NOS

Nitric oxide synthase

NOx

Nitrite plus nitrate

PI3K

Phosphatidyl-inositol-3-kinase

PPARγ

Peroxisome proliferator activated receptor γ

T1DM

Type 1 diabetes mellitus

T2DM

Type 2 diabetes mellitus

TNF-α

Tumor necrosis factor-α

WBC

White blood cell(s)

Copyright information

© Springer Basel AG 2011

Authors and Affiliations

  • András Hrabák
    • 1
  • László Derzbach
    • 2
  • Ildikó Csuka
    • 1
  • Tamás Bajor
    • 3
  • Anna Körner
    • 2
  1. 1.Department of Medical Chemistry, Molecular Biology and PathobiochemistrySemmelweis UniversityBudapestHungary
  2. 2.1st Department of PediatricsSemmelweis UniversityBudapestHungary
  3. 3.Spinner Hungary Ltd.SzekszárdHungary

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