Inflammation Research

, 60:1013

Amino acids exhibit anti-inflammatory effects in human monocytic leukemia cell line, THP-1 cells

Authors

    • Department of PediatricsYamaguchi University Graduate School of Medicine
  • Takashi Ichiyama
    • Department of PediatricsYamaguchi University Graduate School of Medicine
  • Ichiro Sonaka
    • Marketing DepartmentAjinomoto Pharma Co., Ltd.
  • Ayami Ohsaki
    • Department of PediatricsYamaguchi University Graduate School of Medicine
  • Reiji Hirano
    • Department of PediatricsYamaguchi University Graduate School of Medicine
  • Yasuhiro Haneda
    • Department of PediatricsYamaguchi University Graduate School of Medicine
  • Reiji Fukano
    • Department of PediatricsYamaguchi University Graduate School of Medicine
  • Masami Hara
    • Faculty of Human Life Sciences, Department of Food and Health SciencesJissen Women’s University
  • Susumu Furukawa
    • Department of PediatricsYamaguchi University Graduate School of Medicine
    • Faculty of Human Life Sciences, Department of Food and Health SciencesJissen Women’s University
Original Research Paper

DOI: 10.1007/s00011-011-0362-1

Cite this article as:
Hasegawa, S., Ichiyama, T., Sonaka, I. et al. Inflamm. Res. (2011) 60: 1013. doi:10.1007/s00011-011-0362-1

Abstract

Objective

The elemental diet is one of the effective therapies for inflammatory bowel disease. However, the mechanism remains unclear, and there have never been reports about the inhibitory effects of amino acids in human monocytes/macrophages. We investigated the inhibitory effects of amino acids on cytokine production or expression of adhesion molecules that are involved in inflammatory diseases, in human monocytes/macrophages.

Methods

We examined the inhibitory effects of cysteine, histidine or glycine on the induction of nuclear factor-κB (NF-κB) activation, expression of intracellular adhesion molecule-1 (ICAM-1, CD54) and production of interleukin-8 (IL-8) in THP-1 cells, a human monocytic leukemia cell line, and peripheral blood mononuclear cells (PBMCs) stimulated with tumor necrosis factor-α (TNF-α).

Results

Cysteine, histidine and glycine significantly reduced the activation of NF-κB in THP-1 cells stimulated with TNF-α. In addition, cysteine and histidine significantly inhibited the expression of ICAM-1 and production of IL-8 in THP-1 cells and PBMCs.

Conclusions

Our results suggest that cysteine and histidine exhibit anti-inflammatory effects in THP-1 cells, and may be responsible for the efficacy of treatment in inflammatory bowel diseases.

Keywords

Amino acidICAM-1 (CD54)IL-8Monocyte/macrophagesNF-κB

Copyright information

© Springer Basel AG 2011