Inflammation Research

, Volume 60, Issue 9, pp 817–822

Folic acid inhibits lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages by suppressing MAPKs and NF-κB activation

Original Research Paper

DOI: 10.1007/s00011-011-0337-2

Cite this article as:
Feng, D., Zhou, Y., Xia, M. et al. Inflamm. Res. (2011) 60: 817. doi:10.1007/s00011-011-0337-2



The aim of the present study was to investigate the effects of folic acid on the inflammatory responses to lipopolysaccharide (LPS) in RAW264.7 cells and the signal transduction pathways involved.


RAW264.7 macrophages were used. The production of nitric oxide (NO) was determined by the Griess test. The protein levels and mRNA expression of tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) were determined by ELISA and RT-PCR, respectively. The phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear nuclear factor-kappa B (NF-κB) p65 protein were analyzed by Western blotting.


Folic acid inhibited LPS-induced production of NO, TNF-α and IL-1β with decreased mRNAs of inducible nitric oxide synthase (iNOS), TNF-α and IL-1β. Further study showed that folic acid inhibited the LPS-induced phosphorylation of MAPKs and the nuclear translocation of NF-κB.


Folic acid inhibits the inflammatory response of RAW 264.7 cells to LPS through inhibiting the MAPKs and NF-κB pathways.


Folic acid Mitogen-activated protein kinases Nitric oxide Tumor necrosis factor-alpha Interleukin-1 beta 

Copyright information

© Springer Basel AG 2011

Authors and Affiliations

  1. 1.Department of Preventive Medicine, School of Public HealthSun Yat-sen UniversityGuangzhouPeople’s Republic of China
  2. 2.Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public HealthSun Yat-sen University (Northern Campus)GuangzhouPeople’s Republic of China

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