Original Research Paper

Inflammation Research

, Volume 60, Issue 2, pp 153-161

First online:

Toll-like receptor 4 can recognize SapC-DOPS to stimulate macrophages to express several cytokines

  • Kaihua LuAffiliated withImmunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University
  • , Guangfeng ZhaoAffiliated withImmunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University
  • , Hongna LuAffiliated withImmunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University
  • , Shuli ZhaoAffiliated withImmunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University
  • , Yuxian SongAffiliated withImmunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University
  • , Xiaoyang QiAffiliated withJiangsu Changzhou Changji Biotechnology Development Co., Ltd
  • , Yayi HouAffiliated withImmunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing UniversityJiangsu Key Laboratory of Molecular Medicine, Nanjing University Email author 

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Abstract

Objective and design

SapC-DOPS is a newly combined compound consisting of saposin C and dioleoylphosphatidylserine (DOPS). Our recent study showed that SapC-DOPS exhibits anti-tumor activity. However, SapC-DOPS has recognition elements of Toll-like receptor (TLR) 2 and TLR4;therefore, we want to know whether SapC-DOPS can induce abnormal immunoreaction via identification TLRs.

Methods

We investigated the capacity of SapC-DOPS to induce cytokines in vivo and in vitro and analyzed the involvement of TLR and NF-kB in these cytokines production.

Results

SapC-DOPS could activate the cytokine production by peripheral macrophages, enhance the expressions of TLR4 and stimulate the NF-κB nuclear translocation. PDTC, an NF-κB inhibitor, could decrease the SapC-DOPS inducible TNF-α and IL-1β production.

Conclusions

SapC-DOPS was similar to LPS in the immune response and may induce the production of cytokines in macrophages via the TLR4 signaling pathway and, at least in part, the alteration of the NF-κB pathway.

Keywords

SapC-DOPS Raw264.7 TLRs NF-κB