Inflammation Research

, Volume 60, Issue 2, pp 153–161

Toll-like receptor 4 can recognize SapC-DOPS to stimulate macrophages to express several cytokines

Authors

  • Kaihua Lu
    • Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical BiotechnologyNanjing University
  • Guangfeng Zhao
    • Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical BiotechnologyNanjing University
  • Hongna Lu
    • Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical BiotechnologyNanjing University
  • Shuli Zhao
    • Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical BiotechnologyNanjing University
  • Yuxian Song
    • Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical BiotechnologyNanjing University
  • Xiaoyang Qi
    • Jiangsu Changzhou Changji Biotechnology Development Co., Ltd
    • Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical BiotechnologyNanjing University
    • Jiangsu Key Laboratory of Molecular MedicineNanjing University
Original Research Paper

DOI: 10.1007/s00011-010-0249-6

Cite this article as:
Lu, K., Zhao, G., Lu, H. et al. Inflamm. Res. (2011) 60: 153. doi:10.1007/s00011-010-0249-6

Abstract

Objective and design

SapC-DOPS is a newly combined compound consisting of saposin C and dioleoylphosphatidylserine (DOPS). Our recent study showed that SapC-DOPS exhibits anti-tumor activity. However, SapC-DOPS has recognition elements of Toll-like receptor (TLR) 2 and TLR4;therefore, we want to know whether SapC-DOPS can induce abnormal immunoreaction via identification TLRs.

Methods

We investigated the capacity of SapC-DOPS to induce cytokines in vivo and in vitro and analyzed the involvement of TLR and NF-kB in these cytokines production.

Results

SapC-DOPS could activate the cytokine production by peripheral macrophages, enhance the expressions of TLR4 and stimulate the NF-κB nuclear translocation. PDTC, an NF-κB inhibitor, could decrease the SapC-DOPS inducible TNF-α and IL-1β production.

Conclusions

SapC-DOPS was similar to LPS in the immune response and may induce the production of cytokines in macrophages via the TLR4 signaling pathway and, at least in part, the alteration of the NF-κB pathway.

Keywords

SapC-DOPSRaw264.7TLRsNF-κB

Copyright information

© Springer Basel AG 2010