Inflammation Research

, Volume 59, Issue 8, pp 587–595

The chondroprotective effects of ferulic acid on hydrogen peroxide-stimulated chondrocytes: inhibition of hydrogen peroxide-induced pro-inflammatory cytokines and metalloproteinase gene expression at the mRNA level

Authors

  • M. P. Chen
    • Institute of Biomedical Engineering, College of Engineering and College of MedicineNational Taiwan University
  • S. H. Yang
    • National Taiwan University Hospital
  • C. H. Chou
    • Institute of Biomedical Engineering, College of Engineering and College of MedicineNational Taiwan University
  • K. C. Yang
    • Institute of Biomedical Engineering, College of Engineering and College of MedicineNational Taiwan University
  • C. C. Wu
    • Institute of Biomedical Engineering, College of Engineering and College of MedicineNational Taiwan University
    • Department of OrthopedicsEn Chu Kong Hospital
  • Y. H. Cheng
    • Institute of Biomedical Engineering, College of Engineering and College of MedicineNational Taiwan University
    • Institute of Biomedical Engineering, College of Engineering and College of MedicineNational Taiwan University
Original Research Paper

DOI: 10.1007/s00011-010-0165-9

Cite this article as:
Chen, M.P., Yang, S.H., Chou, C.H. et al. Inflamm. Res. (2010) 59: 587. doi:10.1007/s00011-010-0165-9

Abstract

Objective

The objective of the study is to evaluate the effect of ferulic acid (FA), an antioxidant from the Chinese herb Dong-Gui [Chinese angelica, Angelica sinensis (Oliv.) Diels], on the regulation of various genes in hydrogen peroxide-stimulated porcine chondrocytes at the mRNA level.

Methods

The effect of FA and the effective concentration of FA on porcine chondrocytes was evaluated by the lactate dehydrogenase, WST-1, crystal violet assay, and a chemical luminescence assay. Gene expression in hydrogen peroxide-stimulated chondrocytes either pre- or post-treated with FA was evaluated by real-time PCR.

Results

Chondrocytes pre-treated with 40 μM FA decreased the hydrogen peroxide-induced interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and MMP-1 and partially restored SOX9 gene expression. Post-treatment with 40 μM FA also decreased the expression of MMP-1 and MMP-13.

Conclusion

FA decreased the hydrogen peroxide-induced IL-1β, TNF-α, MMP-1 and MMP-13 and increased SOX9 gene expression. These findings suggest that FA may prove to be important in the treatment of osteoarthritis. Further research is needed.

Keywords

OsteoarthritisChondrocyteHydrogen peroxideFerulic acidAntioxidant

Copyright information

© Springer Basel AG 2010