The chondroprotective effects of ferulic acid on hydrogen peroxide-stimulated chondrocytes: inhibition of hydrogen peroxide-induced pro-inflammatory cytokines and metalloproteinase gene expression at the mRNA level
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The objective of the study is to evaluate the effect of ferulic acid (FA), an antioxidant from the Chinese herb Dong-Gui [Chinese angelica, Angelica sinensis (Oliv.) Diels], on the regulation of various genes in hydrogen peroxide-stimulated porcine chondrocytes at the mRNA level.
The effect of FA and the effective concentration of FA on porcine chondrocytes was evaluated by the lactate dehydrogenase, WST-1, crystal violet assay, and a chemical luminescence assay. Gene expression in hydrogen peroxide-stimulated chondrocytes either pre- or post-treated with FA was evaluated by real-time PCR.
Chondrocytes pre-treated with 40 μM FA decreased the hydrogen peroxide-induced interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and MMP-1 and partially restored SOX9 gene expression. Post-treatment with 40 μM FA also decreased the expression of MMP-1 and MMP-13.
FA decreased the hydrogen peroxide-induced IL-1β, TNF-α, MMP-1 and MMP-13 and increased SOX9 gene expression. These findings suggest that FA may prove to be important in the treatment of osteoarthritis. Further research is needed.
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- The chondroprotective effects of ferulic acid on hydrogen peroxide-stimulated chondrocytes: inhibition of hydrogen peroxide-induced pro-inflammatory cytokines and metalloproteinase gene expression at the mRNA level
Volume 59, Issue 8 , pp 587-595
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- SP Birkhäuser Verlag Basel
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- Hydrogen peroxide
- Ferulic acid
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- Author Affiliations
- 1. Institute of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, No. 1, Jen Ai Rd., Sec. 1, Taipei, 10051, Taiwan
- 2. National Taiwan University Hospital, No. 7, Chung Shan S. Rd., Taipei, 100, Taiwan
- 3. Department of Orthopedics, En Chu Kong Hospital, No. 399, Fuhsing Rd., San-shia Town, Taipei Hsien, Taiwan