Inflammation Research

, Volume 58, Issue 12, pp 837–844

Circulating blood monocytes traffic to and participate in the periprosthetic tissue inflammation

  • Kai Zhang
  • Tang-Hong Jia
  • David McQueen
  • Wei-Ming Gong
  • David C. Markel
  • Paul H. Wooley
  • Shang-You Yang
Original Research Paper

DOI: 10.1007/s00011-009-0051-5

Cite this article as:
Zhang, K., Jia, TH., McQueen, D. et al. Inflamm. Res. (2009) 58: 837. doi:10.1007/s00011-009-0051-5

Abstract

Objective

To examine the trafficking of human circulating blood monocytes and their influence on the inflammation of periprosthetic tissues using a novel mouse–human chimera model.

Methods

Periprosthetic tissue and bone chips from patients with aseptic prosthetic loosening were implanted into the muscles of immune-deficient SCID mice depleted of host macrophages by periodic intraperitoneal injection of anti-asialo GM1 rabbit sera (ASGM1). Autologous patient peripheral blood monocytes (PBMCs) were labeled with PKH2 fluorescent dye and injected intraperitoneally into the implanted animals. Mice were sacrificed 14 days after PBMC transfusion for molecular and histological analyses.

Results

Patient periprosthetic tissues were well tolerated in SCID mice and preserved a high level of viability. Cell trafficking studies revealed the accumulation of fluorescent PBMC within the xenografts, with total cell counts in the xenografts significantly increased following the systemic PBMC infusion. PBMC infusion also promoted the expression of IL-1, IL-6, TNFα, and RANK within the periprosthetic tissue.

Conclusion

Systemic PBMC migrated to the implanted periprosthetic tissues and contributed to the local inflammation. The data provide evidence that circulating blood monocytes may play a role in pathologic process during aseptic loosening of total joint replacement.

Keywords

Cell migrationInflammatory modelsMacrophages

Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2009

Authors and Affiliations

  • Kai Zhang
    • 1
    • 2
  • Tang-Hong Jia
    • 1
  • David McQueen
    • 2
  • Wei-Ming Gong
    • 1
  • David C. Markel
    • 4
  • Paul H. Wooley
    • 2
    • 3
  • Shang-You Yang
    • 2
    • 3
  1. 1.Shandong University School of MedicineJinanChina
  2. 2.Orthopaedic Research InstituteVia Christi Regional Medical CenterWichitaUSA
  3. 3.Department of Biological SciencesWichita State UniversityWichitaUSA
  4. 4.Department of Orthopaedic SurgeryProvidence HospitalSouthfieldUSA