Inflammation Research

, Volume 58, Issue 8, pp 463–471

In vivo visualization of nitric oxide and interactions among platelets, leukocytes, and endothelium following hemorrhagic shock and reperfusion

Authors

  • Mie Hiratsuka
    • Department of Obstetrics and GynecologyEhime University School of Medicine
    • Department of Obstetrics and GynecologyEhime University School of Medicine
  • Kazuhiko Uematsu
    • Department of Obstetrics and GynecologyEhime University School of Medicine
  • Masaki Kiyomura
    • Department of Obstetrics and GynecologyUwajima City Hospital
  • Masaharu Ito
    • Department of Obstetrics and GynecologyEhime University School of Medicine
Original Research Paper

DOI: 10.1007/s00011-009-0011-0

Cite this article as:
Hiratsuka, M., Katayama, T., Uematsu, K. et al. Inflamm. Res. (2009) 58: 463. doi:10.1007/s00011-009-0011-0

Abstract

Objectives

We examined changes in nitric oxide (NO) distribution in the mesenteric microcirculation after hemorrhagic shock and reperfusion (H/R), and correlated NO production to leukocyte and platelet behavior.

Materials and methods

The behavior of leukocytes and platelets in mesenteric venules was observed by intravital microscopy at 0.5 and 24 h after H/R in male Wistar rats. Transvascular leakage of fluorescein isothiocyanate-labeled albumin was assessed by epi-illumination. The NO-sensitive dye, 4,5-diaminofluorescein diacetate, was used for imaging NO release.

Results

H/R significantly increased vascular albumin leakage and adhesion of leukocytes and platelets (P < 0.05). In H/R 0.5 h rats, NO production in the venular endothelium declined. However, NO production was elevated in H/R 24 h rats in mast cells (P < 0.05). Leukocyte adherence, platelet adherence, and venular permeability were attenuated by iNOS inhibition.

Conclusion

Mesenteric endothelial cell dysfunction after H/R 0.5 h is associated with reduced NO, whereas after H/R 24 h is related to increase NO in mast cells.

Keywords

EndotheliumIntravital microscopyIschemia reperfusionNitric oxide (NO)Platelets

Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2009