Inflammation Research

, Volume 57, Issue 4, pp 189–198

A genome-wide microarray analysis reveals anti-inflammatory target genes of paeonol in macrophages

  • H. Huang
  • E. J. Chang
  • Y. Lee
  • J. S. Kim
  • S. S. Kang
  • H. H. Kim
Article

DOI: 10.1007/s00011-007-7190-3

Cite this article as:
Huang, H., Chang, E.J., Lee, Y. et al. Inflamm. res. (2008) 57: 189. doi:10.1007/s00011-007-7190-3

Abstract.

Objective:

Paeony root has long been used for its anti-inflammatory effects. In this study, the effects of albiflorin, paeoniflorin, and paeonol, compounds from paeony root, on gene expression profiles were examined in macrophages challenged with the inflammation inducer lipopolysaccharide (LPS).

Methods:

The RAW264.7 macrophages were treated with LPS in the presence or absence of albiflorin, paeoniflorin, or paeonol. Global mRNA expression levels were detected by using an oligonucleotide microarray platform covering the mouse whole genome.

Results:

Treatment with LPS caused expression level changes in 1,270 genes by 2 folds or more. Paeonol attenuated the induction level of 355 LPS-responsive genes. Classification of the genes targeted by paeonol according to the Panther group analysis revealed 20 biological processes, 24 molecular functions, and 22 signaling pathways. The Panther signaling pathways highly affected by paeonol included the ‘inflammation mediated by chemokine and cytokine signaling’, ‘interleukin signaling’, and ‘Toll receptor signaling’.

Conclusion:

Our results demonstrate that paeonol has extensive inhibitory effects on the regulation of inflammation associated gene expression by LPS in macrophages. In addition, the predominant effect of paeonol among the tested compounds suggests that paeonol may be a major ingredient for the anti-inflammatory effect of paeony root.

Key words:

Gene profilingPaeonolPaeoniflorinAlbiflorinMacrophageLipopolysaccharideRAW264.7Microarray

Abbreviations:

CCL

chemokine (C-C motif) ligand

CXCL

chemokine (C-X-C motif) ligand

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

IL

interleukin

IVT

in vitro transcription

LPS

lipopolysaccharide

MCP

monocyte chemotactic protein

PCR

polymerase chain reaction

Ptgs

prostaglandin-endoperoxide synthase

RT

reverse transcription

TLR

Toll-like receptor

TNF

tumor necrosis factor

Supplementary material

11_2007_7190_MOESM1_ESM.pdf (147 kb)
ESM (PDF 147 kb)

Copyright information

© Birkhaueser 2008

Authors and Affiliations

  • H. Huang
    • 1
  • E. J. Chang
    • 1
  • Y. Lee
    • 1
  • J. S. Kim
    • 2
  • S. S. Kang
    • 2
  • H. H. Kim
    • 1
  1. 1.Department of Cell and Developmental Biology, BK21 Program, and DRISeoul National University, School of DentistrySeoulKorea
  2. 2.Natural Products Research Institute and College of PharmacySeoul National UniversitySeoulKorea