Role of lymphocytes in the course of murine zymosan-induced peritonitis
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Objective and Design:
To investigate a putative role of lymphocytes in a murine model of zymosan peritonitis.
Material or Subjects:
Rag-deficient mice (KO) and their counterparts (WT) (13 animals in each group).
Mice were injected i. p. with zymosan (2 mg/ml, 0.5 ml/mouse) and sacrificed either 30 min or 6 h post-treatment.
At 30 min of inflammation vascular permeability was assessed by peritoneal leakage of i. v. injected Evans blue. At 6 h of peritonitis leukocyte numbers were estimated (Turk’s staining), and MMP-2 and -9 presence (zymography). Levels of inflammatory mediators were evaluated by either ELISA (PGE2, KC) or Cytometric Bead Array (IL-6, IL-10, MCP-1, IFN-γ, TNF-α, and IL-12p70). The Amount of nitric oxide (NO) was measured by the Greiss reaction. Differences between WT and KO mice were analyzed by Student’s t-test (p ≤0.05).
During zymosan peritonitis, there was a decreased production of IFN-α (p = 0.03) and IL-10 (p = 0.03) and elevated synthesis of NO (p = 0.0001) in KO mice compared with WT controls. Despite this, no alterations in major events of peritonitis (vascular permeability and neutrophil infiltration) were detected in KO mice.
Lymphocytes do not have a significant impact on zymosan peritonitis in mice.
- Role of lymphocytes in the course of murine zymosan-induced peritonitis
Volume 57, Issue 6 , pp 272-278
- Cover Date
- Print ISSN
- Online ISSN
- SP Birkhäuser Verlag Basel
- Additional Links
- Peritoneal inflammation
- Industry Sectors
- Author Affiliations
- 1. Department of Evolutionary Immunobiology, Institute of Zoology, Jagiellonian University, ul. Ingardena 6, PL-30-060, Krakow, Poland
- 2. Laboratory for Molecular Immunology, German Cancer Research Center, Heidelberg, Germany