Inflammation Research

, Volume 56, Issue 5, pp 204–209

The adenosine A2A receptor agonist CGS 21680 fails to ameliorate the course of dextran sulphate-induced colitis in mice

  • Z. Selmeczy
  • B. Csóka
  • P. Pacher
  • E. S. Vizi
  • G. Haskó
Article

DOI: 10.1007/s00011-006-6150-7

Cite this article as:
Selmeczy, Z., Csóka, B., Pacher, P. et al. Inflamm. res. (2007) 56: 204. doi:10.1007/s00011-006-6150-7

Abstract.

Objective:

In this study we investigated the effect of CGS 21680 (2-p-(2-Carboxyethyl)phenethylamino-5-N-ethylcarboxamidoadenosine hydrochloride), an adenosine A2A receptor agonist, in a model of dextran sulphate sodium (DSS)-induced colitis.

Methods:

NMRI mice were fed 5 % (w/v) DSS, and were treated intraperitoneally with 0.5 mg/kg CGS 21680 or vehicle for 10 days. Changes of bodyweight, colon length, the incidence of rectal bleeding, levels of macrophage inflammatory protein (MIP)-1α, MIP-2, interferon γ, interleukin (IL)-1β, IL-12 and tumour necrosis factor-α from homogenates of colon biopsies, and the release of [3H]acetylcholine (ACh) from longitudinal muscle strip were determined.

Results:

DSS significantly decreased bodyweight, colon length, and it increased the incidence of rectal bleeding and levels of MIP-1α, MIP-2 and IL-1β compared to DSS-untreated animals. CGS 21680 had no effect on these changes. No change could be observed in release of ACh in DSS-induced colitis with or without CGS 21680.

Conclusion:

In summary, CGS 21680 is ineffective in ameliorating DSS-induced colitis in mice.

Keywords:

Dextran sulphate Colitis Adenosine A2A receptor CGS 21680 

Copyright information

© Birkhäuser Verlag, Basel 2007

Authors and Affiliations

  • Z. Selmeczy
    • 1
  • B. Csóka
    • 1
  • P. Pacher
    • 2
  • E. S. Vizi
    • 1
  • G. Haskó
    • 1
    • 3
  1. 1.Department of Pharmacology, Institute of Experimental MedicineHungarian Academy of SciencesBudapestHungary
  2. 2.Laboratory of Physiological Studies, National Institute on Alcohol Abuse and AlcoholismNational Institutes of HealthBethesdaUSA
  3. 3.Department of SurgeryUMDNJ-New Jersey Medical SchoolNewarkUSA

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