Inflammation Research

, Volume 55, Issue 11, pp 511–515

E5564 (Eritoran) inhibits lipopolysaccharide-induced cytokine production in human blood monocytes

Original Paper

DOI: 10.1007/s00011-006-6057-3

Cite this article as:
Czeslick, E., Struppert, A., Simm, A. et al. Inflamm. res. (2006) 55: 511. doi:10.1007/s00011-006-6057-3


Objective and design

In this ex vivo laboratory study, we investigated the effects of E5564 (eritoran), a toll-like receptor 4-directed endotoxin antagonist, on intracellular expression of interleukin (IL)-6 and tumor necrosis factor (TNF)-α in lipopolysaccharide (LPS)-stimulated human monocytes assessed by flow cytometry.

Material and method

Whole blood samples from 10 healthy volunteers (average age: 32  ±  2 years) were pre-incubated with 0.001, 0.003, 0.01, 0.03, 0.1, 0.3, 1 and 10 ng/ml E5564 for 45 min and after this stimulated with LPS (0.2 ng/ml), a dose we found to be the most effective for stimulation. Samples were incubated for 3 h at 37°C and 5% CO2. Intracellular expression of IL-6 and TNF-α was assessed by flow cytometry.


Our investigation showed that E5564 (0.03 ng/ml up to 10 ng/ml) caused a dose-dependent inhibitory effect on IL-6 and TNF-α production in LPS-stimulated human monocytes.


The results of this investigation led us to conclude that E5564 has a remarkable LPS inhibitory activity manifested via down-regulation of the intracellular generation of pro-inflammatory cytokines IL-6 and TNF-α in human monocytes.

Key words.

E5564Proinflammatory cytokinesMonocytesFlow cytometry

Copyright information

© Birkhäuser Verlag, Basel 2006

Authors and Affiliations

  • E. Czeslick
    • 1
  • A. Struppert
    • 1
  • A. Simm
    • 2
  • A. Sablotzki
    • 1
  1. 1.Department of Anesthesiology and Critical Care MedicineMartin Luther University HalleHalle/SaaleGermany
  2. 2.Department of Cardiac and Thoracic SurgeryMartin Luther University HalleHalleGermany