Inflammation Research

, Volume 55, Issue 3, pp 99–107

Inflammation-induced modulation of cellular galectin-1 and -3 expression in a model of rat peritonitis

  • C. D. Gil
  • D. Cooper
  • G. Rosignoli
  • M. Perretti
  • S. M. Oliani
Original Research Papers

DOI: 10.1007/s00011-005-0059-4

Cite this article as:
Gil, C.D., Cooper, D., Rosignoli, G. et al. Inflamm. res. (2006) 55: 99. doi:10.1007/s00011-005-0059-4

Abstract.

Objective and design: To investigate the effect of galectin-1 (Gal-1) and −3 (Gal-3) on leukocyte migration and analyze the expression of both galectins in inflammatory cells using a model of rat peritonitis.

Material or Subjects: Sprague-Dawley rats (n = 4 per group).

Treatment: Peritonitis was induced in animals through intraperitoneal injection of carrageenin (1.5 mg/kg) and rat mesenteries were analyzed at different time points (0, 4, 24 and 48h). For pharmacological treatment, rats received intravenous injection of Gal-1 or -3 (3μg/kg) followed by carrageenin.

Methods: Western blotting and immunoelectron microscopy analysis. Statistical analysis was performed using ANOVA followed by Bonferroni test.

Results: Pharmacological treatment with Gal-1, but not Gal-3, inhibited (~50 %) leukocyte recruitment into the peritoneal cavity at 4 h time-point. In this early phase, immunogold staining of mesenteries showed a diminished Gal-3 expression in degranulated mast cells and Gal-1 in transmigrated neutrophils (~20 % reduction compared to intravascular cells). In the later phases (24 and 48h), leukocyte turnover was associated with augmented Gal-1 expression in neutrophils and macrophages and Gal-3 in mast cells and macrophages.

Conclusions: These results point to a balanced expression of cell-associated-Gal-1/Gal-3 and might impact on the development of new therapeutic strategies for inflammatory diseases.

Keywords.

In vivo inflammation Mast cell Neutrophil Endothelial cell Macrophage 

Copyright information

© Birkhäuser Verlag, Basel 2006

Authors and Affiliations

  • C. D. Gil
    • 2
    • 3
  • D. Cooper
    • 4
  • G. Rosignoli
    • 4
  • M. Perretti
    • 4
  • S. M. Oliani
    • 1
    • 3
  1. 1.Department of Biology, Instituto de Biociências, Letras e Ciências Exatas (IBILCE)University of São Paulo State (UNESP)São José do Rio PretoBrazil
  2. 2.Department of AnatomyFaculty of Medicine (FAMERP)São José do Rio PretoBrazil
  3. 3.Post-Graduation in MorphologySão Paulo School of Medicine – UNIFESPSão PauloBrazil
  4. 4.The William Harvey Research InstituteQueen Mary School of Medicine and DentistryLondonUK