An immunoglobulin agent (IVIG) inhibits NF-κB activation in cultured endothelial cells of coronary arteries in vitro
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- Ichiyama, T., Ueno, Y., Isumi, H. et al. Inflamm. res. (2004) 53: 253. doi:10.1007/s00011-004-1255-3
Objective:Kawasaki disease (KD) is an acute febrile vasculitis of unknown etiology that may lead to cardiovascular disorders. High-dose intravenous immunoglobulin (IVIG) therapy is well established as a standard therapy for KD. Tumor necrosis factor-α (TNF-α) is responsible for the pathogenesis of acute KD. We examined whether or not IVIG inhibits TNF-α-induced activation of transcription factor NF-κB, a factor that is essential for the expression of proinflammatory cytokines, in human coronary artery endothelial cells (CAEC).
Methods:The inhibitory effect of IVIG on NF-κB activation induced by TNF-α was evaluated by Western blot analysis and ELISA. Moreover, the inhibitory effects of IVIG on IκBα degradation, interleukin-6 (IL-6) production, and E-selectin expression induced by TNF-α were evaluated by Western blot analysis, ELISA, and flow cytometry, respectively.
Results:Western blot analysis and ELISA demonstrated that IVIG inhibits NF-κB activation induced by TNF-α in CAEC. Moreover, IVIG inhibited IκBα degradation, IL-6 production, and E-selectin expression induced by TNF-α in CAEC.
Conclusions:The data suggest that IVIG inhibits NF-κB activation induced by TNF-α in CAEC, thereby possibly modulating IL-6 production and E-selectin expression.