Skip to main content
Log in

Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro

Biologia Plantarum

Abstract

In order to establish an efficient system for in vitro plant regeneration of a short day plant Chenopodium rubrum L. and a long day plant Chenopodium murale L., optimum culture conditions for somatic embryogenesis were investigated. The effects of different growth regulators, their combination and their concentrations on somatic embryos induction in different explant types (root, hypocotyl, cotyledon and leaf) were tested. Somatic embryogenesis was induced in both plants on Murashige and Skoog (MS) medium supplemented with sucrose (3 %), agar (0.7 %) and 1 - 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. The largest embryogenic capacity was found in root explants of Chenopodium rubrum on 1 μM 2,4-D and in basal parts of cotyledons in C. murale plants on 10 μM 2,4-D.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

ABA:

abscisic acid

BAP:

6-benzylaminopurine

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

KIN:

6-furfurylaminopurine

PEG:

polyethylene glycol

References

  • Al-Khayri, J.M.: Micropropagation of Spinacia oleracea L. (spinach).-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Vol. 39. Pp. 173–200. Springer-Verlag, Berlin-Heidelberg 1997.

    Google Scholar 

  • Al-Matar, S.M., Al-Khayri, J.M., Brar, M.S., Klingaman, G.L.: In vitro regeneration of long spur barrenwort (Epimedium grandiflorum Morr.) from rachis explants.-In Vitro cell. dev. Biol. Plant 35: 245–248, 1999.

    Google Scholar 

  • Ammirato, P.V.: Embryogenesis.-In: Evans, D.A., Sharp, W.R., Ammirato, P.V., Yamada, Y. (ed.): Handbook of Plant Cell Culture. Vol. 1. Pp. 82–123. MacMillan, New York 1983.

    Google Scholar 

  • Ćulafić, Lj., Budimir, S., Vujičić, R., Nešković, M.: Induction of somatic embryogenesis and embryo development in Rumex acetosella L.-Plant Cell Tisue Organ Culture 11: 133–139, 1987.

    Article  Google Scholar 

  • D ‘Onofrio, C., Morini, S.: Simultaneous regeneration of different morphogenic structures from quince leaves as affected by growth regulator combination and treatment length.-Biol. Plant. 47: 321–325, 2003/4.

    Article  CAS  Google Scholar 

  • Kim, S.H., Kim, S.K.: Effects of auxins and cytokinins on callus induction from leaf blade, petiole, and stem segments of in vitro-grown åSheridan« grape shoots.-J. Plant Biotechnol. 4: 17–21, 2002.

    Google Scholar 

  • Komai, F., Okuse, I., Harada, T.: Somatic embryogenesis and plant regeneration in culture of root segments of spinach (Spinacia oleracea L.).-Plant Sci. 113: 203–208, 1996.

    Article  CAS  Google Scholar 

  • Krekule, J., Macháčková, I., Pavlová, L., Seidlová, F.: Hormonal signals in photoperiodic control of flower initiation.-In: Krekule, J., Seidlová, F. (ed.): Signals in Plant Development. Pp. 145–162. SPB Academic Publishing, The Hague 1989.

    Google Scholar 

  • Mandal, A.K.A., Dutta Gupta, S., Chatterji, A.K.: Factors affecting somatic embryogenesis from cotyledonary explants of safflower.-Biol. Plant. 44: 503–507, 2001.

    Article  Google Scholar 

  • Milivojević, S.: In vitro morphogenesis of Chenopodium rubrum L., a short-day plant and Chenopodium murale L., a long-day plant-MS Thesis. University of Belgrade, Belgrade 2003.

    Google Scholar 

  • Milivojević, S., Mitrović, A., Ćulafić, Lj.: Different morphogenetic patways of Chenopodium rubrum L. regeneration in vitro.-In: Redžić, S., Đug, S. (ed.): Third International Balkan Botanical Congress. P. 349. Faculty of Science of the University of Sarajevo, Sarajevo 2003.

    Google Scholar 

  • Mitrović, A., Živanović, B., Ćulafić, Lj.: The effects of photoperiod, glucose and gibberellic acid on growth in vitro and flowering of Chenopodium murale.-Biol. Plant. 43: 173–177, 2000.

    Article  Google Scholar 

  • Mitrović, A., Živanović, B., Ćulafić, Lj.: Effect of darkness on growth and flowering of Chenopodium rubrum and C. murale plants in vitro.-Biol. Plant. 46: 471–474, 2003.

    Article  Google Scholar 

  • Murashige T., Skoog F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Nešković, M., Vujičić, R., Budimir, S.: Somatic embryogenesis and bud formation from immature embryos of buckwheat (Fagopyrum esculentum Moench.).-Plant Cell Rep. 6: 423–426, 1987.

    Google Scholar 

  • Pavlová, L., Krekule, J.: Fluctuation of free IAA under inductive and non-inductive photoperiods in Chenopodium rubrum.-J. Plant Growth Regul. 2: 94–98, 1984.

    Google Scholar 

  • Raimondi, J.P., Camadro, E.L., Babinec, F.J.: Somatic embryogenesis in Asparagus officinalis L. cv. Argenteuil: interactions between genotype, explant type and growth regulators on callus induction, growth and embryogenic differentiation.-Biocell 25: 147–154, 2001.

    CAS  PubMed  Google Scholar 

  • Sasaki, H., Arato, T., Takahashi, K., Kobori, T.: Regeneration of spinach plant from hypocotyl tissue cultured in vitro.-J. Hokkaido Univ. Educ. 45: 31–35, 1994.

    Google Scholar 

  • Tétu, T., Sangwan, R.S., Sangwan-Norreel, B.S.: Hormonal control of organogenesis and somatic embryogenesis in Beta vulgaris callus.-J. exp. Bot. 38: 506–517, 1987.

    Google Scholar 

  • Xiao, X.G., Branchard, M.: Embryogenesis and plant regeneration of spinach (Spinacia oleracea L.) from hypocotyls segments.-Plant Cell Rep. 13: 69–71, 1993.

    CAS  Google Scholar 

  • Zdravković-Korać, S., Nešković, M.: Organogenesis and somatic embryogenesis in Spinacia oleracea tissue culture.-Arch. Biol. Sci. Belgrade 50: 183–188, 1998.

    Google Scholar 

  • Zdravković-Korać, S., Nešković, M.: Induction and development of somatic embryos from spinach (Spinacia oleracea L.) leaf segments.-Plant Cell Tissue Organ Cult. 55: 109–114, 1999.

    Article  Google Scholar 

  • Živanović, B., Ćulafić, Lj., Filipović, A.: The effects of hormones and saccharides on growth and flowering of green and herbicides-treated Chenopodium rubrum L. plants.-Biol. Plant. 37: 257–264, 1995.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to S. Milivojević.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Milivojević, S., Mitrović, A. & Ćulafić, L. Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro . Biol Plant 49, 35–39 (2005). https://doi.org/10.1007/s00000-005-5039-5

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00000-005-5039-5

Additional key words

Navigation