Journal of Plant Research

, Volume 114, Issue 3, pp 381–385

A Simple Method for Developing Microsatellite Markers using Amplified Fragments of Inter-simple Sequence Repeat (ISSR)

  • Chunlan Lian
  • Zhihua Zhou
  • Taizo Hogetsu
Technical Note

DOI: 10.1007/PL00014001

Cite this article as:
Lian, C., Zhou, Z. & Hogetsu, T. J Plant Res (2001) 114: 381. doi:10.1007/PL00014001

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A primer IP1 designed from the sequenced region at one end of the microsatellite and for nested PCR another primer IP2 based on the sequence between IP1 and the microsatellite were prepared. These two primers were used to determine the other sequence flanking the microsatellite by a “walking” method. With this approach, we developed several microsatellite markers from Salix reinii, Pinus densiflora and Robinia pseudoacacia, respectively. The absence of enrichment processes and screening procedures makes it easier to develop microsatellite markers, and this approach provides an alternative for the development of microsatellite markers in any organism.

Keywords: Inter-simple sequence repeat, Microsatellite isolation, “Walking” sequencing

Copyright information

© The Botanical Society of Japan 2001

Authors and Affiliations

  • Chunlan Lian
    • 1
  • Zhihua Zhou
    • 1
  • Taizo Hogetsu
    • 1
  1. 1.Asian Natural Environmental Science Center, the University of Tokyo, 1–1–8 Midori-cho, Nishi-Tokyo, Tokyo, 188–0002 JapanJP