Specific Detection of Tomato Pathotype of Verticillium dahliae by PCR Assays
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Klebahn is the causal agent of tomato wilt disease. Isolates of V. dahliae can be classified based on pathogenicity to tomato, but the pathotypes are indistinguishable in morphology. We designed PCR primers for specific detection of isolates pathogenic to tomato (tomato pathotype) from the sequences of a pathotype-specific gene, vdt1. With the primer pair Tg5/Tc3, a PCR product (approximately 3.2 kb) specific to tomato pathotype was amplified from the genomic DNA of isolates. Using the primer pair, a tomato pathotype isolate was specifically detected from hypocotyls of inoculated tomato and eggplant. On the other hand, no amplification was observed from non-tomato pathotype isolates of V. dahliae, some other wilt pathogens of tomato and a healthy host plant. Therefore, the primer pair can be useful for pathotype-specific detection of V. dahliae as well as for diagnosis of wilt disease of tomato plant.
- Specific Detection of Tomato Pathotype of Verticillium dahliae by PCR Assays
Journal of General Plant Pathology
Volume 68, Issue 2 , pp 134-140
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- The Phytopathological Society of Japan
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- Key words : Verticillium dahliae, tomato pathotype, pathotype-specific detection, PCR.
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