Journal of General Plant Pathology

, Volume 68, Issue 2, pp 134–140

Specific Detection of Tomato Pathotype of Verticillium dahliae by PCR Assays

Authors

  • Toshiyuki USAMI
    • Faculty of Horticulture, Chiba University, Matsudo 271-8510, Japan
  • Mafumi ABIKO
    • Graduate School of Life Science, Tohoku University, Aoba, Sendai 980-8577, Japan
  • Masahiro SHISHIDO
    • Faculty of Horticulture, Chiba University, Matsudo 271-8510, Japan
  • Yoshimiki AMEMIYA
    • Faculty of Horticulture, Chiba University, Matsudo 271-8510, Japan

DOI: 10.1007/PL00013066

Cite this article as:
USAMI, T., ABIKO, M., SHISHIDO, M. et al. J Gen Plant Pathol (2002) 68: 134. doi:10.1007/PL00013066

Verticillium dahliae

Klebahn is the causal agent of tomato wilt disease. Isolates of V. dahliae can be classified based on pathogenicity to tomato, but the pathotypes are indistinguishable in morphology. We designed PCR primers for specific detection of isolates pathogenic to tomato (tomato pathotype) from the sequences of a pathotype-specific gene, vdt1. With the primer pair Tg5/Tc3, a PCR product (approximately 3.2 kb) specific to tomato pathotype was amplified from the genomic DNA of isolates. Using the primer pair, a tomato pathotype isolate was specifically detected from hypocotyls of inoculated tomato and eggplant. On the other hand, no amplification was observed from non-tomato pathotype isolates of V. dahliae, some other wilt pathogens of tomato and a healthy host plant. Therefore, the primer pair can be useful for pathotype-specific detection of V. dahliae as well as for diagnosis of wilt disease of tomato plant.

Key words : Verticillium dahliae, tomato pathotype, pathotype-specific detection, PCR.

Copyright information

© The Phytopathological Society of Japan 2002