Journal of General Plant Pathology

, Volume 68, Issue 2, pp 118–127

Functional Characterization of Citrus Polygalacturonase-inhibiting Protein

Authors

  • Sarunya NALUMPANG
    • United Graduate School of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan
  • Yukie GOTOH
    • Laboratory of Plant Pathology, Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan
  • Hiroyuki TSUBOI
    • Dainippon Ink and Chemicals, Incorporated, Agrichemicals Division, Central Research Laboratories, Sakura 285-8668, Japan
  • Kenji GOMI
    • United Graduate School of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan
  • Hiroyuki YAMAMOTO
    • Laboratory of Plant Pathology, Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan
  • Kazuya AKIMITSU
    • Laboratory of Plant Pathology, Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan

DOI: 10.1007/PL00013064

Cite this article as:
NALUMPANG, S., GOTOH, Y., TSUBOI, H. et al. J Gen Plant Pathol (2002) 68: 118. doi:10.1007/PL00013064

A cDNA encoding a polygalacturonase-inhibiting protein gene (SaiPGIPA) was identified from the citrus cultivar Sainumphung (Citrus sp.), one of the most popular cultivars in northern Thailand. SaiPGIPA was expressed in Escherichia coli cells, and the functional properties of citrus PGIP were analyzed. The PGIP fusion protein inhibited by a maximum of about 60% of the endopolygalacturonase activity, and a mixture of the PGIP and fungal endopoly-galacturonase released oligogalacturonides from polygalacturonic acid. The mixture containing the oligogalactur-onides, endopolygalacturonase and PGIP induced expression of the PGIP gene and a chalcone synthase gene in citrus leaves. The mixture also induced resistance in cucumber leaves against Colletotrichum lagenarium.

Key words : polygalacturonase-inhibiting protein, citrus, Alternaria alternate, polygalacturonase, Sainumphung, rough lemon.

Copyright information

© The Phytopathological Society of Japan 2002