Journal of General Plant Pathology

, Volume 68, Issue 1, pp 57–61

A cDNA Encoding Polygalacturonase-inhibiting Protein Induced in Citrus Leaves by Polygalacturonase of Alternaria citri

  • Yukie GOTOH
  • Sarunya NALUMPANG
  • Atsunori ISSHIKI
  • Takami UTSUMI
  • Kenji GOMI
  • Hiroyuki YAMAMOTO
  • Kazuya AKIMITSU

DOI: 10.1007/PL00013054

Cite this article as:
GOTOH, Y., NALUMPANG, S., ISSHIKI, A. et al. J Gen Plant Pathol (2002) 68: 57. doi:10.1007/PL00013054

A cDNA encoding the polygalacturonase-inhibiting protein gene (RlemPGIPl) was identified from rough lemon (Citrus jambhiri Lush.). The deduced amino acid sequence is 46-75% identical to other plant PGIPs. The transcripts of the PGIP gene were weakly but constitutively expressed in leaves and increased in leaves within 30 min after wounding or inoculation with nonpathogenic Alternaria alternata or A. citri. However, expression of the PGIP and chalcone synthase genes did not increase after inoculation with an endopolygalacturonase (endoPG)-disrupted mutant of A. citri. These results indicate that endoPG may act as an elicitor for induction of these genes in citrus, most likely caused by PGIP interactions.

Key words : Alternaria alternata, Alternaria citri, citrus, polygalacturonase-inhibiting protein, polygalacturonase, rough lemon.

Copyright information

© The Phytopathological Society of Japan 2002

Authors and Affiliations

  • Yukie GOTOH
    • 1
  • Sarunya NALUMPANG
    • 2
  • Atsunori ISSHIKI
    • 2
  • Takami UTSUMI
    • 1
  • Kenji GOMI
    • 2
  • Hiroyuki YAMAMOTO
    • 1
  • Kazuya AKIMITSU
    • 1
  1. 1.Laboratory of Plant Pathology, Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761–0795, JapanJP
  2. 2.United Graduate School of Agriculture, Kagawa University, Miki, Kagawa 761–0795, JapanJP