Molecular and General Genetics MGG

, Volume 263, Issue 1, pp 1–11

The 51,409-bp R-plasmid pTP10 from the multiresistant clinical isolate Corynebacterium striatum M82B is composed of DNA segments initially identified in soil bacteria and in plant, animal, and human pathogens

  • A. Tauch
  • S. Krieft
  • J. Kalinowski
  • A. Pühler
ORIGINAL PAPER

DOI: 10.1007/PL00008668

Cite this article as:
Tauch, A., Krieft, S., Kalinowski, J. et al. Mol Gen Genet (2000) 263: 1. doi:10.1007/PL00008668

Abstract

The 51,409-bp DNA sequence of the multiresistance plasmid pTP10 from the gram-positive opportunistic human pathogen Corynebacterium striatum M82B has been determined. Fully automated genome interpretation led to the identification of 47 ORFs. Analysis of the genetic organization of pTP10 suggests that the plasmid is composed of eight DNA segments, the boundaries of which are represented by transposons and insertion sequences. The DNA segments of pTP10 are highly similar to (1) a plasmid-encoded erythromycin resistance region from the human pathogen Corynebacterium diphtheriae; (2) a chromosomal DNA region from Mycobacterium tuberculosis; (3) a plasmid-encoded chloramphenicol resistance region from the soil bacterium Corynebacterium glutamicum; (4) transposable elements from phytopathogenic gram-negative Pseudomonas, Xanthomonas and Erwinia species; and (5) a plasmid-encoded aminoglycoside resistance region from the gram-negative fish pathogen Pasteurella piscicida. The complete DNA sequence of pTP10 provides genetic information regarding the mechanisms of resistance to 16 antimicrobial agents that belong to six structural classes. In addition, the mosaic structure of pTP10 represents the evolutionary consolidation into a single plasmid molecule of antimicrobial resistances from microorganisms found in different habitats by means of mobile elements, resulting in the generation of a multiresistant bacterium that can infect humans.

Key wordsCorynebacterium striatumMultiresistanceTransposable elementGene transferPlasmid evolution

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • A. Tauch
    • 1
  • S. Krieft
    • 1
  • J. Kalinowski
    • 1
  • A. Pühler
    • 1
  1. 1.Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, 33501 Bielefeld, Germany E-mail: Andreas.Tauch@genetik.uni-bielefeld.de Tel.: +49-521-1065604; Fax: +49-521-1065626DE