Lung

, Volume 177, Issue 5, pp 311–319

Elevation of Anti-cytokeratin 19 Antibody in Sera of the Patients With Idiopathic Pulmonary Fibrosis and Pulmonary Fibrosis Associated With Collagen Vascular Disorders

  • J.  Fujita
  • N.  Dobashi
  • Y.  Ohtsuki
  • I.  Yamadori
  • T.  Yoshinouchi
  • T.  Kamei
  • M.  Tokuda
  • S.  Hojo
  • H.  Okada
  • J.  Takahara

DOI: 10.1007/PL00007649

Cite this article as:
Fujita, J., Dobashi, N., Ohtsuki, Y. et al. Lung (1999) 177: 311. doi:10.1007/PL00007649

Abstract.

It has been suggested that cytokeratin 19 is expressed in regenerated bronchoepithelial cells in patients with pulmonary fibrosis, and serum cytokeratin 19 fragment is elevated in patients with pulmonary fibrosis. We hypothesized that serum antibodies to cytokeratin 19 may be formed in patients with pulmonary fibrosis. To prove the existence of anti-cytokeratin 19 antibodies in patients' sera, human recombinant cytokeratin 19 was stained with patients' sera by a Western immunoblot. Then, we tried to establish an enzyme-linked immunosorbent assay to quantitate anti-cytokeratin 19 antibody in the sera of patients with idiopathic pulmonary fibrosis (IPF) and pulmonary fibrosis associated with collagen vascular disorders (PF-CVD). We demonstrated the anti-cytokeratin 19 antibody in patient' sera by a Western immunoblot. In patients with IPF and PF-CVD, significantly high anti-cytokeratin 19 antibody was demonstrated compared with normal volunteers, patients with chronic bronchitis, and patients with pneumonia. These results suggest that anti-cytokeratin 19 antibody may have played a role in the process of lung injury in pulmonary fibrosis.

Key words: Antibody—Cytokeratin 19—Idiopathic pulmonary fibrosis—Pulmonary fibrosis associated with collagen vascular disorder. 

Copyright information

© 1999 Springer-Verlag New York Inc.

Authors and Affiliations

  • J.  Fujita
    • 1
  • N.  Dobashi
    • 1
  • Y.  Ohtsuki
    • 2
  • I.  Yamadori
    • 3
  • T.  Yoshinouchi
    • 4
  • T.  Kamei
    • 5
  • M.  Tokuda
    • 1
  • S.  Hojo
    • 1
  • H.  Okada
    • 1
  • J.  Takahara
    • 1
  1. 1.First Department of Internal Medicine, Kagawa Medical University, Kagawa, JapanJP
  2. 2.Department of Pathology, Kochi Medical School, Kochi, JapanJP
  3. 3.Department of Pathology, Okayama University Medical School, Okayama, JapanJP
  4. 4.Department of Respiratory Medicine, Gamagohri City Hospital, Aichi, JapanJP
  5. 5.Department of Internal Medicine, Kagawa Prefectural Central Hospital, Kagawa, JapanJP