Acta Neuropathologica

, Volume 99, Issue 1, pp 55–62

Local activation of the complement system in endoneurial microvessels of diabetic neuropathy

Authors

  • G. B. Rosoklija
    • Department of Pathology (Division of Neuropathology), College of Physicians and Surgeons, Columbia University, PH 15th Floor Stem, Room 124, 630 West 168th Street, New York, NY 10032, USA Tel.: +1-212-305-4531, Fax: +1-212-305-4548
  • A. J. Dwork
    • Department of Pathology (Division of Neuropathology), College of Physicians and Surgeons, Columbia University, PH 15th Floor Stem, Room 124, 630 West 168th Street, New York, NY 10032, USA Tel.: +1-212-305-4531, Fax: +1-212-305-4548
  • D. S. Younger
    • Neurological Institute, College of Physicians and Surgeons, Columbia University, New York, N.Y., USA
  • G. Karlikaya
    • Department of Pathology (Division of Neuropathology), College of Physicians and Surgeons, Columbia University, PH 15th Floor Stem, Room 124, 630 West 168th Street, New York, NY 10032, USA Tel.: +1-212-305-4531, Fax: +1-212-305-4548
  • N. Latov
    • Neurological Institute, College of Physicians and Surgeons, Columbia University, New York, N.Y., USA
  • A. P. Hays
    • Department of Pathology (Division of Neuropathology), College of Physicians and Surgeons, Columbia University, PH 15th Floor Stem, Room 124, 630 West 168th Street, New York, NY 10032, USA Tel.: +1-212-305-4531, Fax: +1-212-305-4548
Regular paper

DOI: 10.1007/PL00007406

Cite this article as:
Rosoklija, G., Dwork, A., Younger, D. et al. Acta Neuropathol (2000) 99: 55. doi:10.1007/PL00007406

Abstract

Quantitative immunocytochemical analysis of complement proteins (CP) was performed on sural nerve biopsies from 15 patients with diabetic neuropathy (DN) and 18 nondiabetic patients with other forms of chronic neuropathy (ON). The mean age of the patients and the pathological severity of the neuropathy were similar in both groups. The percentage of patients that expressed strongly immunoreactive CP in the walls of endoneurial microvessels was significantly greater in DN than in ON for all proteins tested. C3d neoantigen was expressed in 100% of DN cases compared with 17% of ON; and membrane attack complex (MAC), C5b-9 neoantigen, in 93% of DN and 17% of ON. In the cases with DN, 81% of endoneurial microvessels, as identified by the endothelial marker, Ulex europaeus, contained C5b-9 neoantigen deposits, compared with 22% in those of ON, and the staining in DN was significantly more intense. Expression of the neoantigens of C3d and C5b-9 in nerve implies local activation of the complement system. In DN, activation of the complement pathway and formation of the MAC could injure blood vessels and adversely affect the circulation in the endoneurium.

Key words Diabetes mellitus Diabetic neuropathy Complement components Membrane attack complex Microvessels

Copyright information

© Springer-Verlag Berlin Heidelberg 2000