Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 359, Issue 1, pp 65–70

Defaecation, intestinal fluid accumulation and motility in rodents: implications of cannabinoid CB1 receptors

  • Angelo A. Izzo
  • Nicola Mascolo
  • Francesca Borrelli
  • F. Capasso
Original article

DOI: 10.1007/PL00005325

Cite this article as:
Izzo, A., Mascolo, N., Borrelli, F. et al. Naunyn-Schmiedeberg's Arch Pharmacol (1999) 359: 65. doi:10.1007/PL00005325
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Abstract

We have studied the effect of SR141716A (0.1–5 mg/kg, i.p.), a cannabinoid CB1 receptor antagonist, and WIN (0.1–5 mg/kg, i.p.), a cannabinoid receptor agonist, on acute defaecation and gastrointestinal transit in mice and on intraluminal fluid accumulation in the rat small intestine. SR141716A increased while WIN 55,212-2 decreased defaecation, gastrointestinal transit and fluid accumulation. A per se non-effective dose of SR141716A (0.3 mg/kg) counteracted the inhibitory effect of WIN 55,212-2 (1 mg/kg) on gastrointestinal functions studied. The effect of SR141716 on both intestinal fluid accumulation in rats and gastrointestinal transit in mice was inhibited by atropine (1 mg/kg, i.p.), but not by hexamethonium (1 mg/kg, s.c.), SR140333 (20 µg/kg, i.p.) or SR48968 (20 µg/kg, i.p.), antagonists of NK1 and NK2 receptors, respectively. These results suggest that intestinal fluid accumulation and motility are inhibited by endogenous cannabinoid(s) acting at the cannabinoid CB1 receptors. This effect may be mediated by mechanisms involving muscarinic cholinoceptors.

Key words Cannabinoid Defaecation Small intestine Intestinal motility Intestinal secretion 

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Angelo A. Izzo
    • 1
  • Nicola Mascolo
    • 2
  • Francesca Borrelli
    • 1
  • F. Capasso
    • 1
  1. 1.Department of Experimental Pharmacology, University of Naples “Federico II”, via D. Montesano 49, I-80131 Naples, Italy Fax: +39-81-7486403IT
  2. 2.School of Pharmacy, University of Salerno, Fisciano, ItalyIT

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