Cellular and Molecular Life Sciences CMLS

, Volume 58, Issue 3, pp 403–419

Transposase and cointegrase: specialized transposition proteins of the bacterial insertion sequence IS21 and related elements

  • B. Berger
  • D. Haas

DOI: 10.1007/PL00000866

Cite this article as:
Berger, B. & Haas, D. CMLS, Cell. Mol. Life Sci. (2001) 58: 403. doi:10.1007/PL00000866

Abstract:

The bacterial insertion sequence IS21 shares with many insertion sequences a two-step, reactive junction transposition pathway, for which a model is presented in this review: a reactive junction with abutted inverted repeats is first formed and subsequently integrated into the target DNA. The reactive junction occurs in IS21-IS21 tandems and IS21 minicircles. In addition, IS21 shows a unique specialization of transposition functions. By alternative translation initiation, the transposase gene codes for two products: the transposase, capable of promoting both steps of the reactive junction pathway, and the cointegrase, which only promotes the integration of reactive junctions but with higher efficiency. This review also includes a survey of the IS21 family and speculates on the possibility that other members present a similar transpositional specialization.

Key words: Transposase; cointegrase; insertion sequence; transposition; IS21 family; IS30; IS911; retrovirus. 

Copyright information

© Birkhäuser Verlag, 2001

Authors and Affiliations

  • B. Berger
    • 1
  • D. Haas
    • 1
  1. 1.Laboratoire de Biologie Microbienne, Université de Lausanne, CH-1015 Lausanne (Switzerland), Fax +41216925635, e-mail: Dieter.Haas@lbm.unil.chCH