Cellular and Molecular Life Sciences CMLS

, Volume 58, Issue 2, pp 215–224

Nitrate and nitrite transport in bacteria

Authors

  • J. W. B. Moir*
    • Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN (United Kingdom), Fax +44 114 272 8697, e-mail: j.moir@sheffield.ac.uk
  • N. J. Wood
    • Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN (United Kingdom), Fax +44 114 272 8697, e-mail: j.moir@sheffield.ac.uk

DOI: 10.1007/PL00000849

Cite this article as:
Moir*, J. & Wood, N. CMLS, Cell. Mol. Life Sci. (2001) 58: 215. doi:10.1007/PL00000849

Abstract.

The topological arrangements of nitrate and nitrite reductases in bacteria necessitate the synthesis of transporter proteins that carry the nitrogen oxyanions across the cytoplasmic membrane. For assimilation of nitrate (and nitrite) there are two types of uptake system known: ABC transporters that are driven by ATP hydrolysis, and secondary transporters reliant on a proton motive force. Proteins homologous to the latter type of transporter are also involved in nitrate and nitrite transport in dissimilatory processes such as denitrification. These proteins belong to the NarK family, which is a branch of the Major Facilitator Superfamily. The mechanism and substrate specificity of transport via these proteins is unknown, but is discussed in the light of sequence analysis of members of the NarK family. A hypothesis for nitrate and nitrite transport is proposed based on the finding that there are two distinct types of NarK.

Key words. Nitrate; nitrite; transporter; dissimilatory; assimilatory; NarK.

Copyright information

© Birkhäuser Verlag Basel, 2001