Cellular and Molecular Life Sciences CMLS

, Volume 58, Issue 2, pp 179–193

The coordination and function of the redox centres of the membrane-bound nitrate reductases

Authors

  • F. Blasco*
    • Laboratoire de Chimie Bactérienne, IBSM, CNRS, 31 chemin Joseph Aiguier, F-13402 Marseille Cedex 20 (France), Fax +33 04 91 71 89 14, e-mail: blasco@ibsm.cnrs-mrs.fr
  • B. Guigliarelli
    • Laboratoire de Bioénergétique et Ingénierie des Protéines, IBSM, CNRS, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20 (France)
  • A. Magalon
    • Laboratoire de Chimie Bactérienne, IBSM, CNRS, 31 chemin Joseph Aiguier, F-13402 Marseille Cedex 20 (France), Fax +33 04 91 71 89 14, e-mail: blasco@ibsm.cnrs-mrs.fr
  • M. Asso
    • Laboratoire de Bioénergétique et Ingénierie des Protéines, IBSM, CNRS, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20 (France)
  • G. Giordano
    • Laboratoire de Chimie Bactérienne, IBSM, CNRS, 31 chemin Joseph Aiguier, F-13402 Marseille Cedex 20 (France), Fax +33 04 91 71 89 14, e-mail: blasco@ibsm.cnrs-mrs.fr
  • R. A. Rothery
    • Department of Biochemistry, 474 Medical Science Building, University of Alberta, Edmonton, Alberta T6G 2H7 (Canada)

DOI: 10.1007/PL00000846

Cite this article as:
Blasco*, F., Guigliarelli, B., Magalon, A. et al. CMLS, Cell. Mol. Life Sci. (2001) 58: 179. doi:10.1007/PL00000846

Abstract.

Under anaerobic conditions and in the presence of nitrate, the facultative anaerobe Escherichia coli synthesises an electron-transport chain comprising a primary dehydrogenase and the terminal membrane-bound nitrate reductase A (NarGHI). This review focuses on recent advances obtained on the structure and function of the three protein subunits of membrane-bound nitrate reductases. We discuss a global architecture for the Mo-bisMGD-containing subunit (NarG) and a coordination model for the four [Fe–S] centres of the electron-transfer subunit (NarH) and for the two b-type haems of the anchor subunit NarI.

Key words. Nitrate reductase; molybdenum cofactor; [Fe–S] centres; haems.

Copyright information

© Birkhäuser Verlag Basel, 2001